Three-way fluorescence data and
multivariate calibrationbased on parallel factor analysis (PARAFAC) are combined for the si
multaneous quantitation of three fluoroquinolone anitibiotics (norfloxacin, enoxacin, and ofloxacin) in human serum samples. The three analytes canbe adequately determined with limits of detection of 0.2,3.0, and 0.5
g L
-1, respectively, with mini
mum experimental effort. The selected analytical methodology fullyexploits the so-called second-order advantage of theemployed three-way data, allowing obtaining individualconcentrations of calibrated analytes in the presence ofany number of uncalibrated (serum) components. Incontrast to PARAFAC, less satisfactory results wereobtained with a
multidimensional partial least-squares(
nPLS) model trained with the same calibration set.