Photosensitizer-Loaded Branched Polyethylenimine-PEGylated Ceria Nanoparticles for Imaging-Guided Synchronous Photochemotherapy

详细信息    查看全文

• 作者：Zhang-You Yang ; Hong Li ; Yi-Ping Zeng ; Yu-Hui Hao ; Cong Liu ; Jing Liu ; Wei-Dong Wang ; Rong Li
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：November 4, 2015
• 年：2015
• 卷：7
• 期：43
• 页码：24218-24228
• 全文大小：712K
• ISSN：1944-8252

文摘

A multifunctional theranostic platform based on photosensitizer (chlorin e6, Ce6)-loaded branched polyethylenimine-PEGylated ceria nanoparticles (PPCNPs鈥揅e6) was created for the development of effective cancer treatments involving the use of imaging-guided synchronous photochemotherapy. PPCNPs鈥揅e6 with high Ce6 photosensitizer loading (Ce6: cerium 鈭?0 wt %) significantly enhanced the delivery of Ce6 into cells and its accumulation in lysosomes, remarkably improving photodynamic therapeutic (PDT) efficacy levels compared to those in the administration of free Ce6 at ultralow drug doses (鈭?00 nM). Interestingly, PPCNPs鈥揅e6 efficiently induced HeLa cell death even at low concentrations (鈭?0 渭M) without the use of laser irradiation and exhibit chemocytotoxicity. Inductively coupled plasma mass spectrometry (ICP-MS) and biology transmission electron microscopy (Bio-TEM) analyses demonstrated that ceria nanoparticles enter cells abundantly and accumulate in lysosomes or large vesicles. We then evaluated the effects of the different materials on lysosomal integrity and function, which revealed that PPCNPs鈥揅e6 catastrophically impaired lysosomal function compared to results with PPCNPs and Ce6. Studies of apoptosis revealed greater induction of apoptosis by PPCNPs鈥揅e6 treatment. This multifunctional nanocarrier also exhibited a high degree of solubility and stability in aqueous solutions, suggesting its applicability for extensive biomedical application.