Electrochemical Detection of Peroxynitrite Using a Biosensor Based on a Conducting Polymer−Manganese Ion Complex

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• 作者：Wei Choon Alvin Koh ; Jung Ik Son ; Eun Sang Choe ; Yoon-Bo Shim
• 刊名：Analytical Chemistry
• 出版年：2010
• 出版时间：December 15, 2010
• 年：2010
• 卷：82
• 期：24
• 页码：10075-10082
• 全文大小：298K
• 年卷期：v.82,no.24(December 15, 2010)
• ISSN：1520-6882

文摘

A peroxynitrite (ONOO⁻) biosensor has been developed through the preparation of a new manganese−[poly-2,5-di-(2-thienyl)-1H-pyrrole)-1-(p-benzoic acid)] (Mn−pDPB) complex. DPB monomer was first synthesized and polymerized for the purpose of providing a polymer backbone for complex formation with Mn²⁺ ion. The Mn−pDPB complex was characterized via Magnetomotive Force (MMF) simulation, X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry. The complex selectively enhanced the reduction process of ONOO⁻ which was used as the analytical signal for chronoamperometric detection. A polyethyleneimmine (PEI) layer was coated on the complex surface to increase selectivity and stability. The chronoamperometric calibration plot showed the hydrodynamic range of 2.0 × 10⁻⁸−5.0 × 10⁻⁷ M. The detection limit was determined to be 1.9 (±0.2) × 10⁻⁹ M based on S/N = 3. The microbiosensor, fabricated on a 100 μm diameter Pt tip, was applied in a real rat plasma sample for the detection of spiked concentrations of ONOO⁻. The reliability and long-term stability of the microbiosensor was also examined with YPEN-1 cells in vitro, and the results shown were promising.