The carbon isotope ratios (
13C) of 160 beers from around the world ranged from -27.3 to -14.9,primarily due to variation in the percentage of C
3 or C
4 plant carbon in the final product. Thirty-onepercent of beers had a carbon signature of C
3 plants (barley, rice, etc.), whereas the remaining 69%contained some C
3-C
4 mixture (mean of mixtures, 39 ± 11% C
4 carbon). Use of C
4 carbon (corn,cane sugar, etc.) was not confined to beers from any particular region (Pacific Rim, Mexico, Brazil,Europe, Canada, and the United States). However, the
13C of European beers indicated mostly C
3plant carbon. In contrast, U.S. and Canadian beers contained either only C
3 or C
3-C
4 mixtures;Brazilian, Mexican, and Pacific Rim beers were mostly C
3-C
4 mixtures. Among different lagers, U.S.-style lagers generally contained more C
4 carbon than did imported pilsners. Among different ales,those brewed by large high-production breweries contained significant proportions of C
4 carbon, whileC
4 carbon was not detected in microbrewery or home-brew ales. Furthermore, inexpensive beersgenerally contained more C
4 carbon than expensive beers.Keywords: Carbon isotope ratio;
13C; beer; adjuncts; C
4; C
3