Molecular Engineering of Acoustic Protein Nanostructures
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- 作者：Anupama Lakshmanan ; Arash Farhadi ; Suchita P. Nety ; Audrey Lee-Gosselin ; Raymond W. Bourdeau ; David Maresca ; Mikhail G. Shapiro
- 刊名：ACS Nano
- 出版年：2016
- 出版时间：August 23, 2016
- 年：2016
- 卷：10
- 期：8
- 页码：7314-7322
- 全文大小：532K
- 年卷期：0
- ISSN：1936-086X
文摘
Ultrasound is among the most widely used biomedical imaging modalities, but has limited ability to image specific molecular targets due to the lack of suitable nanoscale contrast agents. Gas vesicles—genetically encoded protein nanostructures isolated from buoyant photosynthetic microbes—have recently been identified as nanoscale reporters for ultrasound. Their unique physical properties give gas vesicles significant advantages over conventional microbubble contrast agents, including nanoscale dimensions and inherent physical stability. Furthermore, as a genetically encoded material, gas vesicles present the possibility that the nanoscale mechanical, acoustic, and targeting properties of an imaging agent can be engineered at the level of its constituent proteins. Here, we demonstrate that genetic engineering of gas vesicles results in nanostructures with new mechanical, acoustic, surface, and functional properties to enable harmonic, multiplexed, and multimodal ultrasound imaging as well as cell-specific molecular targeting. These results establish a biomolecular platform for the engineering of acoustic nanomaterials.