Cell-Selective Bioorthogonal Metabolic Labeling of RNA
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- 作者：Kim Nguyen ; Michael Fazio ; Miles Kubota ; Sarah Nainar ; Chao Feng ; Xiang Li ; Scott X. Atwood ; Timothy W. Bredy ; Robert C. Spitale
- 刊名：Journal of the American Chemical Society
- 出版年：2017
- 出版时间：February 15, 2017
- 年：2017
- 卷：139
- 期：6
- 页码：2148-2151
- 全文大小：280K
- ISSN：1520-5126
文摘
Stringent chemical methods to profile RNA expression within discrete cellular populations remains a key challenge in biology. To address this issue, we developed a chemical–genetic strategy for metabolic labeling of RNA. Cell-specific labeling of RNA can be profiled and imaged using bioorthogonal chemistry. We anticipate that this platform will provide the community with a much-needed chemical toolset for cell-type specific profiling of cell-specific transcriptomes derived from complex biological systems.