Protein-Functionalized Polymer Brushes

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• 作者：Stefano Tugulu ; Anke Arnold ; India Sielaff ; Kai Johnsson ; and Harm-Anton Klok
• 刊名：Biomacromolecules
• 出版年：2005
• 出版时间：May 2005
• 年：2005
• 卷：6
• 期：3
• 页码：1602 - 1607
• 全文大小：253K
• 年卷期：v.6,no.3(May 2005)
• ISSN：1526-4602

文摘

A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based ona combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformateactivation of the surface hydroxyl groups, and subsequent O⁶-benzylguanine (BG) functionalization. TheBG-functionalized brushes are used to chemoselectively immobilize O⁶-alkylguanine-DNA-alkyltransferase(AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushesare attractive candidates for the development of protein microarrays.