Population Study of Sizes and Components of Self-Reproducing Giant Multilamellar Vesicles

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• 作者：Taro Toyota ; Katsuto Takakura ; Yoshiyuki Kageyama ; Kensuke Kurihara ; Naoto Maru ; Kiyoshi Ohnuma ; Kunihiko Kaneko ; Tadashi Sugawara
• 刊名：Langmuir
• 出版年：2008
• 出版时间：April 1, 2008
• 年：2008
• 卷：24
• 期：7
• 页码：3037 - 3044
• 全文大小：475K
• 年卷期：v.24,no.7(April 1, 2008)
• ISSN：1520-5827

文摘

Population analysis of a system of self-reproducing giant multilamellar vesicles (GMVs) was carried out by meansof flow cytometry. The multidimensional distribution of forward light scattering (FS), side light scattering (SS), andfluorescence (FL) intensities originating from each GMV provided information about changes in a population composedof 10⁴ vesicles. FS-FL dot plots indicated that, after the addition of the membrane precursor, the size distributionof the newly generated vesicles was nearly the same as that of the original, but the catalyst content was reduced. Thisresult can be interpreted as evidence for the occurrence of the self-reproduction of GMVs. Moreover, the new GMVsrecovered the amount of catalyst to the initial value, keeping their size distribution constant, when a solution of thecatalyst was added to the new GMVs. These results are the first experimental evidence for a novel phenomenon onGMV size distribution during their self-reproducing cycle.