pH Responsive Janus-like Supramolecular Fusion Proteins for Functional Protein Delivery

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• 作者：Seah Ling Kuan ; David Y. W. Ng ; Yuzhou Wu ; Christina F枚rtsch ; Holger Barth ; Mikheil Doroshenko ; Kaloian Koynov ; Christoph Meier ; Tanja Weil
• 刊名：Journal of the American Chemical Society
• 出版年：2013
• 出版时间：November 20, 2013
• 年：2013
• 卷：135
• 期：46
• 页码：17254-17257
• 全文大小：278K
• 年卷期：v.135,no.46(November 20, 2013)
• ISSN：1520-5126

文摘

A facile, noncovalent solid-phase immobilization platform is described to assemble Janus-like supramolecular fusion proteins that are responsive to external stimuli. A chemically postmodified transporter protein, DHSA, is fused with (imino)biotinylated cargo proteins via an avidin adaptor with a high degree of spatial control. Notably, the derived heterofusion proteins are able to cross cellular membranes, dissociate at acidic pH due to the iminobiotin linker and preserve the enzymatic activity of the cargo proteins 尾-galactosidase and the enzymatic subunit of Clostridium botulinum C2 toxin. The mix-and-match strategy described herein opens unique opportunities to access macromolecular architectures of high structural definition and biological activity, thus complementing protein ligation and recombinant protein expression techniques.