文摘
A competitive indirect enzyme-linked immunosorbentassay (ciELISA) using monoclonal antibodies (Mabs)having broad specificity for fluoroquinolone (FQ) antibiotics is described. Four FQs, ciprofloxacin (CIP), enrofloxacin (ENR), norfloxacin (NOR), and ofloxacin (OFL),were conjugated to bovine serum albumin for immunogens and to ovalbumin for coating antigens. A MabC4A9H1 raised against the CIP hapten exhibited highcross-reactivity (35-100%) with 12 of 14 FQs anddetected these FQs in a ciELISA below their maximumresidue levels (MRLs) with good sensitivity at 50% bindinginhibition (IC50). The quantitative structure-activity relationship (QSAR) between Mab C4A9H1 and variousFQs by comparative molecular field analysis (CoMFA)showed a high predictive ability with a cross-validationq2 value of 0.866. Using a simple purification process andthe broad-specificity ciELISA adapted for analysis of FQsin chicken muscle, chicken liver, honey, shrimp, andwhole egg samples demonstrated recoveries of 60-93%for CIP, ENR, NOR, OFL, flumequine, and danofloxacin.