Hydrogen-Deuterium Exchange and Selective Labeling of Deprotonated Amino Acids and Peptides in the Gas Phase

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• 作者：Zhixin Tian ; Lev Lis ; Steven R. Kass
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：January 9, 2008
• 年：2008
• 卷：130
• 期：1
• 页码：8 - 9
• 全文大小：78K
• 年卷期：v.130,no.1(January 9, 2008)
• ISSN：1520-5126

文摘

Hydrogen-deuterium exchange reactions of deprotonated amino acids and small peptides were studied. Selective labeling can be carried out at the -amino group of lysine (2 of 4 labile hydrogens undergo exchange with CF₃CH₂OD) and the guanidine side chain of arginine (3 of 6 labile hydrogens undergo exchange with CH₃CH₂OD and C₆H₅CH₂OD). Differential labeling of peptides also was accomplished, and the extent of H/D exchange is dependent on the amino acids which are present as well as their order in the chain.