Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus
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- 作者：Phuong Dinh Kim ; Vladim铆r 艩a拧ek ; Lenka Burketov谩 ; Jana 膶op铆kov谩 ; Andriy Synytsya ; Barbora Jind艡ichov谩 ; Olga Valentov谩
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：June 5, 2013
- 年：2013
- 卷：61
- 期：22
- 页码：5207-5214
- 全文大小：323K
- 年卷期：v.61,no.22(June 5, 2013)
- ISSN：1520-5118
文摘
Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans, a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with 伪- or 尾-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (58 mol %), mannose (22 mol %), and galactose (18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue 伪-d-Glcp-(1鈫?)-尾-d-Galf-(1鈫?)-伪-d-Manp-(1鈫?)-l-Ser. Terminal 伪-d-Glcp and (1鈫?)-尾-d-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrates induce defense response in B. napus plants.