A Peptide-Based Dendrimer That Enhances the Splice-Redirecting Activity of PNA Conjugates in Cells

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• 作者：Fatouma Said Hassane ; Gabriela D. Ivanova ; Eleonora Bolewska-Pedyczak ; Rachida Abes ; Andrey A. Arzumanov ; Michael J. Gait ; Bernard Lebleu ; Jean Garipy
• 刊名：Bioconjugate Chemistry
• 出版年：2009
• 出版时间：August 19, 2009
• 年：2009
• 卷：20
• 期：8
• 页码：1523-1530
• 全文大小：234K
• 年卷期：v.20,no.8(August 19, 2009)
• ISSN：1520-4812

文摘

The full therapeutic potential of oligonucleotide (ON)-based agents has been hampered by cellular delivery challenges. Cell-penetrating peptides (CPP) represent promising delivery vectors for nucleic acids, and their potential has recently been evaluated using a functional splicing redirection assay, which capitalizes on the nuclear delivery of splice-correcting steric-block ON analogues such as peptide nucleic acids (PNA). Despite encouraging in vitro and in vivo data with arginine-rich CPP-steric block conjugates, mechanistic studies have shown that entrapment within the endosome/lysosome compartment after endocytosis remains a limiting factor. Previous work from our group has shown that CPP oligomerization greatly improves cellular delivery and increases transfection of plasmid DNA. We now report the chemical synthesis and the evaluation of multivalent CPP−PNA constructs incorporating monomeric (p53^mono) and dendrimer-like tetrameric (p53^tet) forms of the p53 tetramerization domain containing peptide, a 10 arginine CPP domain (R10), and a splice redirecting PNA (PNA705). These CPP−PNA conjugates were termed R10p53^tet-PNA705 and R10p53^mono-PNA705, referring to their oligomerization state. The present study demonstrates that the splicing redirection efficiency of PNA705 is much greater in the context of the tetrameric R10p53^tet-PNA705 construct than for the monomeric and occurs at nanomolar concentrations, demonstrating that multivalency is an important factor in delivering PNA into cells.