Purification and Characterization of a Corrinoid-Compound in an Edible Cyanobacterium Aphanizomenon flos-aquae as a Nutritional Supplementary Food
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- 作者：Emi Miyamoto ; Yuri Tanioka ; Motoyuki Nakao ; Florin Barla ; Hiroshi Inui ; Tomoyuki Fujita ; Fumio Watanabe ; Yoshihisa Nakano
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：December 13, 2006
- 年：2006
- 卷：54
- 期：25
- 页码：9604 - 9607
- 全文大小：53K
- 年卷期：v.54,no.25(December 13, 2006)
- ISSN：1520-5118
文摘
The vitamin B12 concentration of the dried cells of Aphanizomenon flos-aquae was determined byboth microbiological method with Lactobacillus delbrueckeii ATCC7830 and chemiluminescencemethod with intrinsic factor. The Aphanizomenon cells contained 616.3 ± 30.3 
g (n = 4) of vitaminB12 per 100 g of the dried cells by the microbiological method. The values determined with thechemiluminescence method, however, were only about 5.3% of the values determined by themicrobiological method. A corrinoid-compound was purified from the dried cells and characterized.The purified corrinoid-compound was identified as pseudovitamin B12 (an inactive corrinoid-compoundfor humans) by silica gel 60 TLC, C18 reversed-phase HPLC, ultraviolet-visible spectroscopy, and1H NMR spectroscopy. The results suggest that the Aphanizomenon cells are not suitable for use asa vitamin B12 source, especially in vegans.
g (n = 4) of vitaminB12 per 100 g of the dried cells by the microbiological method. The values determined with thechemiluminescence method, however, were only about 5.3% of the values determined by themicrobiological method. A corrinoid-compound was purified from the dried cells and characterized.The purified corrinoid-compound was identified as pseudovitamin B12 (an inactive corrinoid-compoundfor humans) by silica gel 60 TLC, C18 reversed-phase HPLC, ultraviolet-visible spectroscopy, and1H NMR spectroscopy. The results suggest that the Aphanizomenon cells are not suitable for use asa vitamin B12 source, especially in vegans.