Participation of two carbonic anhydrases of the alpha family in photosynthetic reactions in Arabidopsis thaliana

详细信息    查看全文

• 作者：E. M. Zhurikova ; L. K. Ignatova ; N. N. Rudenko ; V. A. Mudrik…
• 关键词：Key wordsphotosynthesis ; carbonic anhydrase ; arabidopsis ; gene expression ; mutants
• 刊名：Biochemistry (Moscow)
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：81
• 期：10
• 页码：1182-1187
• 全文大小：260 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Bioorganic Chemistry
  Microbiology
  Biomedicine
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3040
• 卷排序：81

文摘

The expression of genes of two carbonic anhydrases (CA) belonging to the a-family, α-CA2 and α-CA4 (according to the nomenclature in N. Fabre et al. (2007) Plant Cell Environ., 30, 617-629), was studied in arabidopsis (Arabidopsis thaliana, var. Columbia) leaves. The expression of the At2g28210 gene coding α-CA2 decreased under increase in plant illumination, while the expression of the At4g20990 gene coding α-CA4 increased. Under conditions close to optimal for photosynthesis, in plants with gene At2g28210 knockout, the effective quantum yield of photosystem 2 and the light-induced accumulation of hydrogen peroxide in leaves were lower than in wild type plants, while the coefficient of non-photochemical quenching of leaf chlorophyll a fluorescence and the rate of CO₂ assimilation in leaves were higher. In plants with At4g20990 gene knockout, the same characteristics changed in opposite ways relative to wild type. Possible mechanisms of the participation of αa-CA2 and α-CA4 in photosynthetic reactions are discussed, taking into account that protons can be either consumed or released in the reactions they catalyze.