Study of Forced Degradation Behaviour of Brinzolamide Using LC–ESI–Q-TOF and In Silico Toxicity Prediction
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- 作者：Chiguru Vishnuvardhan ; Debasish Swain ; Roshan Borkar ; S. Gananadhamu…
- 关键词：Brinzolamide ; Stress testing ; Degradation products ; UHPLC–MS/MS ; In silico toxicity
- 刊名：Chromatographia
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：79
- 期：19-20
- 页码：1293-1308
- 全文大小：1,511 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Analytical Chemistry
Organic Chemistry
Pharmacy
Biochemistry
Plant Sciences
Measurement Science and Instrumentation - 出版者：Vieweg Verlag
- ISSN：1612-1112
- 卷排序：79
文摘
Brinzolamide (BZA) is a topical ophthalmic drug which is generally used to lower the intraocular pressure during glaucoma. It was subjected to forced degradation studies under hydrolytic (acidic, basic), oxidative, photolytic and thermal stress conditions; the drug degraded significantly in hydrolytic and oxidative conditions, leading to the formation of seven degradation products in total. It was stable on exposure to light and dry heat in the solid state. An ultra-performance liquid chromatography (UPLC) method was developed on a Waters CSH phenyl hexyl column (100 × 2.1 mm, 1.7 µm), using gradient elution of 0.1 % formic acid and methanol as mobile phase. The method was extended to quadrupole time-of-flight mass spectrometry (Q-TOF–MS) for the structural characterisation. All degradation products were comprehensively characterised by UHPLC–ESI/MS/MS experiments. The most probable mechanisms for the formation of degradation products were also proposed. In silico toxicity of the drug and its degradation products was determined using TOPKAT toxicity prediction software.