Microbial succession in white button mushroom production systems from compost and casing to a marketable packed product

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• 作者：Nazareth A. Siyoum ; Karen Surridge ; Elna J. van der Linde…
• 关键词：Agaricus bisporus ; Bacteria ; Casing ; Compost ; Fungi ; Microbial succession ; Yeasts
• 刊名：Annals of Microbiology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：66
• 期：1
• 页码：151-164
• 全文大小：1,162 KB
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• 作者单位：Nazareth A. Siyoum (1)
  Karen Surridge (2)
  Elna J. van der Linde (3)
  Lise Korsten (1)
  
  1. Department of Plant Science, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa
  2. Department of Plant Production and Soil Science, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa
  3. Plant Protection Research Institute, Agricultural Research Council, P/Bag X134, Queenswood, 0121, Pretoria, South Africa
  
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

The aim of the study was to investigate microbial succession in the mushroom supply chain from compost, casing to fruit body formation and mushroom growth to the point of harvest, packing and point of sale. The microbial population dynamics of compost, casing and mushrooms were determined using a plate count technique, denaturing gradient gel electrophoresis (DGGE) and sequencing of 16S and 18S rDNA. Plating revealed greater abundance of bacteria, fungi and yeasts in mushroom compost compared to casing and fresh mushroom samples. The viable count method also showed that bacteria and yeasts increased significantly after harvest and during cold storage. Sequencing revealed a more diverse culturable bacterial population in casing and on the mushrooms than in the compost. Phylogenetic analysis revealed a general trend of grouping of species from the same sources. In contrast, a higher microbial diversity was recorded in compost when using the DGGE method, which reflects both cultural and non-culturable microorganisms. For compost and casing bacteria studied using DGGE, several species formed separate lineages, demonstrating highly diverse communities in these samples. Fungi were shown to be less abundant and less diverse compared to bacteria and yeasts. The study provides baseline knowledge of microbial populations and succession trends in mushroom production systems using viable and non-viable methods. The information provided in this study may be useful for microbial ecology studies and to identify and develop biocontrol systems for pathogen control during production or to enhance pinning stimulation by knowing when to apply Pseudomonas spp. to ensure increased yield. Finally, an insight is provided into microbial survival during cold storage and marketing of mushrooms. Potential antagonistic populations known to prevent spoilage, quality deterioration and extend shelf life are listed in this paper. Keywords Agaricus bisporus Bacteria Casing Compost Fungi Microbial succession Yeasts