Thein vitro effect of theophylline on 17伪, 20脽-dihydroxy-4-pregnen-3-one-induced germinal vesicle breakdown in the catfish,Clarias batrachus

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• 作者：Shail K. Chaube ; S. Haider
• 关键词：Oocyte maturation ; catfish ; phosphodiesterase inhibitor ; 17伪 ; 20脽 ; dihydroxy ; 4 ; pregnen ; 3 ; one ; cAMP
• 刊名：Journal of Biosciences
• 出版年：1995
• 出版时间：June 1995
• 年：1995
• 卷：20
• 期：3
• 页码：439-444
• 全文大小：
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  DeManno D A and Goetz F W 1987b Steroid-induced final maturation in brook trout (Salvelinus fontinalis) oocytesin vitro: The effects of forskolin and phosphodiesterase inhibitors;Biol. Reprod. 36 1321鈥?332PubMed CrossRef
  Finet B, Jalabert B and Garg S K 1988 Effect of defolliculation and 17伪-hydroxy,20脽-dihydro-progesterone on cyclic AMP level in full-grown oocytes of the rainbow trout,Salmo gairdneri;Gamete Res. 19 241鈥?52PubMed CrossRef
  Goetz F W 1983 Hormonal control of oocyte final maturation and ovulation in fishes; inFish physiology (eds) W S Hoar, D J Randall and E M Donaldson (New York: Academic Press) Vol. 9B, pp 117鈥?70
  Goetz F W and Hennessy T 1984 Thein vitro effects of phosphodiesterase inhibitors on 17伪,20脽-dihydroxy-4-pregnen-3-one-induced germinal vesicle breakdown in brook troutSalvelinus Fontinalis oocytes;Comp. Biochem. Physiol. A77 785鈥?86CrossRef
  Haider S and Chaube S K 1995 Changes in total cAMP levels during oocyte maturation in the catfish,Clarias batrachus;Comp. Biochem. Physiol. (in press)
  Haider S and Rao N V 1992 Oocyte maturation inClarias batrachus. III. Purification and characterization of maturation-inducing steroid;Fish Physiol. Biochem. 9 505鈥?12CrossRef
  Jalabert B and Finet B 1986 Regulation of oocyte maturation in rainbow trout,Salmo gairdneri: Role of cyclic AMP in the mechanism of action of the maturation inducing steroid (MIS), 17伪 hydroxy, 20尾- dihydroprogesterone;Fish Physiol. Biochem. 2 65鈥?4CrossRef
  Jalabert B, Fostier A, Breton B and Weil C 1991 Oocyte maturation in vertebrates; inVertebrate Endocrinology: Fundamentals and Biomedical Implications (eds) Peter K T Pang and M P Schreibman (New York: Academic Press) Vol. 4A, pp 1鈥?96
  Nagahama Y 1987 Endocrine control of oocyte maturation; inHormones and Reproduction in Fishes, Amphibians and Reptiles (eds) D O Norris and R E Jones (New York: Plenum Press) pp 171鈥?02
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  Trant J M and Thomas P 1988 Structure-activity relationships of steroids in inducing germinal vesicle breakdown of Atlantic croaker oocytesin vitro;Gen. Comp. Endocrinol. 71 307鈥?17PubMed CrossRef
  Upadhyaya N and Haider S 1986 Germinal vesicle breakdown in oocytes of catfish,Mystus vittatus (Bloch): Relativein vitro effectiveness of estradiol-17脽, androgens, corticosteroids, progesterone and other pregnene derivatives;Gen. Comp. Endocrinol. 63 70鈥?6PubMed CrossRef
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• 作者单位：Shail K. Chaube (1)
  S. Haider (1)
  
  1. Department of Zoology, Banaras Hindu University, 221 005, Varanasi, India
  
• 刊物类别：Life Sciences, general; Biomedicine general; Zoology; Plant Sciences; Microbiology; Cell Biology;
• 刊物主题：Life Sciences, general; Biomedicine general; Zoology; Plant Sciences; Microbiology; Cell Biology;
• 出版者：Springer India
• ISSN：0973-7138

文摘

The effects of theophylline (a phosphodiesterase inhibitor) and cAMP on 17伪, 20脽-dihydroxy-4-pregnen-3-one-induced germinal vesicle breakdown was investigatedin vitro in catfish (Clarias batrachus) oocytes. Folliculated oocytes incubated with 17伪, 20脽-dihydroxy-4-pregnen-3-one at the concentration of 1 渭g/ml induced 93.2 卤 2.23% germinal vesicle breakdown. When the oocytes were prestimulated with 17伪,20脽-dihydroxy-4-pregnen-3-one for 6 h and then treated with different concentrations of theophylline, there was a significant drop in the frequency of germinal vesicle breakdown at the concentrations 2.0, 1.5 and 1.0 mM. However, theophylline was found to be incapable of inhibiting germinal vesicle breakdown at its lowest concentration (0.5 inM). In the time course study, significant inhibition of germinal vesicle breakdown was recorded when 1 mM theophylline was added up to 30 h of 17伪,20脽-dihydroxy-4-pregnen-3-one Stimulation but the inhibitory effect of theophylline gradually (time dependent manner) declined if the stimulatory time of 17伪,20脽-dihydroxy-4-pregnen-3-one was increased. A similar inhibition of germinal vesicle breakdown was also recorded with various concentrations of cAMP. Except 0.5 mM, all the higher concentrations of cAMP significantly inhibited 17伪,20脽-dihydroxy-4-pregnen-3-one induced germinal vesicle breakdown. Keywords Oocyte maturation catfish phosphodiesterase inhibitor 17伪,20脽-dihydroxy-4-pregnen-3-one cAMP