Low-expressed testis-specific calcium-binding protein CBP86-IV (CABYR) is observed in idiopathic asthenozoospermia

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• 作者：Shulin Shen ; Jinzi Wang ; Jihong Liang ; Chunhui Zhu
• 关键词：Idiopathic asthenozoospermia ; Molecular marker ; Testis ; specific calcium ; binding protein CBP86 ; IV
• 刊名：World Journal of Urology
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：33
• 期：5
• 页码：633-638
• 全文大小：664 KB
• 参考文献：1.Kretsinger RH (1976) Calcium-binding proteins. Annu Rev Biochem 45:239-66View Article PubMed
  2.Yanez M, Gil-Longo J, Campos-Toimil M (2012) Calcium binding proteins. Adv Exp Med Biol 740:461-82View Article PubMed
  3.Mosior M, Epand RM (1997) Protein kinase C: an example of a calcium-regulated protein binding to membranes (review). Mol Membr Biol 14(2):65-0View Article PubMed
  4.Iismaa TP, Shine J (1992) G protein-coupled receptors. Curr Opin Cell Biol 4(2):195-02View Article PubMed
  5.Nejatbakhsh N, Feng ZP (2011) Calcium binding protein-mediated regulation of voltage-gated calcium channels linked to human diseases. Acta Pharmacol Sin 32(6):741-48View Article PubMed Central PubMed
  6.Hurley MJ, Brandon B, Gentleman SM, Dexter DT (2013) Parkinson’s disease is associated with altered expression of CaV1 channels and calcium-binding proteins. Brain 136(Pt 7):2077-097View Article PubMed
  7.Bernstein HG, Blazejczyk M, Rudka T, Gundelfinger ED, Dobrowolny H, Bogerts B, Kreutz MR, Kuznicki J, Wojda U (2005) The Alzheimer disease-related calcium-binding protein Calmyrin is present in human forebrain with an altered distribution in Alzheimer’s as compared to normal ageing brains. Neuropathol Appl Neurobiol 31(3):314-24View Article PubMed
  8.Kim YH, Jha KN, Mandal A, Vanage G, Farris E, Snow PL, Klotz K, Naaby-Hansen S, Flickinger CJ, Herr JC (2005) Translation and assembly of CABYR coding region B in fibrous sheath and restriction of calcium binding to coding region A. Dev Biol 286(1):46-6View Article PubMed
  9.Chiriva-Internati M, Cobos E, Da Silva DM, Kast WM (2008) Sperm fibrous sheath proteins: a potential new class of target antigens for use in human therapeutic cancer vaccines. Cancer Immun 8:8PubMed Central PubMed
  10.Tseng YT, Hsia JY, Chen CY, Lin NT, Chong PC, Yang CY (2011) Expression of the sperm fibrous sheath protein CABYR in human cancers and identification of alpha-enolase as an interacting partner of CABYR-a. Oncol Rep 25(4):1169-175PubMed
  11.Hong CY, Chiang BN, Turner P (1984) Calcium ion is the key regulator of human sperm function. Lancet 2(8417-418):1449-451View Article PubMed
  12.Li YF, He W, Mandal A, Kim YH, Digilio L, Klotz K, Flickinger CJ, Herr JC (2011) CABYR binds to AKAP3 and Ropporin in the human sperm fibrous sheath. Asian J Androl 13(2):266-74View Article PubMed Central PubMed
  13.Li YF, He W, Kim YH, Mandal A, Digilio L, Klotz K, Flickinger CJ, Herr JC (2010) CABYR isoforms expressed in late steps of spermiogenesis bind with AKAPs and ropporin in mouse sperm fibrous sheath. Reprod Biol Endocrinol 8:101View Article PubMed Central PubMed
  14.Hsu HC, Lee YL, Cheng TS, Howng SL, Chang LK, Lu PJ, Hong YR (2005) Characterization of two non-testis-specific CABYR variants that bind to GSK3beta with a proline-rich extensin-like domain. Biochem Biophys Res Commun 329(3):1108-117View Article PubMed
  15.World Health Organization (2010) WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction, 4th edn. Cambridge University Press, Cambridge, New York
  16.Martinez-Heredia J, de Mateo S, Vidal-Taboada JM, Ballesca JL, Oliva R (2008) Identification of proteomic differences in asthenozoospermic sperm samples. Hum Reprod 23(4):783-91View Article PubMed
  17.Shen S, Wang J, Liang J, He D (2013) Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia. World J Urol 31(6):1395-401View Article PubMed
  18.Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402-08View Article PubMed
  19.Naaby-Hansen S, Mandal A, Wolkowicz MJ, Sen B, Westbrook VA, Shetty J, Coonrod SA, Klotz KL, Kim YH, Bush LA et al (2002) CABYR, a novel calcium-binding tyrosine phosphorylation-regulated fibrous sheath protein involved in capacitation. Dev Biol 242(2):236-54View Article PubMed
  20.Turner RM, Musse MP, Mandal A, Klotz K, Jayes FC, Herr JC, Gerton GL, Moss SB, Chemes HE (2001) Molecular genetic analysis of two human sperm fibrous sheath proteins, AKAP4 and AKAP3, in men with dysplasia of the fibrous sheath. J Androl 22(2):302-15PubMed
  
• 作者单位：Shulin Shen (1)
  Jinzi Wang (2)
  Jihong Liang (1)
  Chunhui Zhu (1)
  
  1. Department of Andrology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China
  2. Agricultural College, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530004, China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Urology and Andrology
  Nephrology
  Oncology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1433-8726

文摘

Objectives To investigate the expression level of testis-specific calcium-binding protein CBP86-IV in normal and asthenozoospermic human sperm. Methods The total RNA was extracted from human sperm, and target cDNA was obtained by reverse transcription–polymerase chain reaction. Then the cDNA was used for quantitative PCR analysis and cloned into the prokaryotic expression vector pET-28a, respectively. The fusion protein was induced and expressed as inclusion body which was used to produce the polyclonal antibody against TSCBP86-IV. The protein expression level of TSCBP86-IV from normal human sperm and idiopathic asthenozoospermic samples was detected by the purified antibody. Results The experimental results showed that the protein expression of TSCBP86-IV was reduced in idiopathic asthenozoospermia and consistent with the transcriptional changing tendency which was detected by quantitative PCR analysis. Conclusions The stable and reliable change of TSCBP86-IV may be taken as a new molecular marker for clinical diagnosis of idiopathic asthenozoospermia.