T-2 Toxin Alters the Levels of Collagen II and Its Regulatory Enzymes MMPs/TIMP-1 in a Low-Selenium Rat Model of Kashin-Beck Disease

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• 作者：Xiaorong Zhou ; Haojie Yang ; Fang Guan ; Senhai Xue…
• 关键词：T ; 2 toxin ; Collagen II ; MMPs/TIMP ; 1 ; Kashin ; Beck disease ; Rat model
• 刊名：Biological Trace Element Research
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：169
• 期：2
• 页码：237-246
• 全文大小：6,659 KB
• 参考文献：1.Mo DX (1987) Pathology of selenium deficiency in Kashin-Beck disease. In: (Eds.) GFCESALEO (ed) Selenium in biology and medicine: Proceedings of the Third International Symposium on Selenium in Biology and Medicine. Van Nostrand Reinhold, New York, pp 859-866
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  17.Zhou JW, Wang CQ, Zhou JH, Zhang WX (2004) Effects of MMP-3/TIMP-1 in rat ischemia/reperfusion articular cartilage. J Bone Joint Injuries 19:325–327
  
• 作者单位：Xiaorong Zhou (1)
  Haojie Yang (2)
  Fang Guan (3)
  Senhai Xue (4)
  Daiqin Song (5)
  Jinghong Chen (6)
  Zhilun Wang (6)
  
  1. Department of Geriatric Surgery, The Second Hospital of Medical College of Xi’an Jiaotong University, Xi’an, Shaanxi Province, 710004, China
  2. Department of Prevention and Health Care, The Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province, 710004, China
  3. The Faculty of Science, Xi’an Jiao Tong University, Xi’an, Shaanxi Province, 710049, China
  4. Department of Clinical Nutrition, Xijing Hospital, The Fourth Military Medical University, Xian, 710032, China
  5. Institute of Endemic and Chronic Diseases Prevention, Center for Disease Control and Prevention, Zibo, Shandong Province, 255026, China
  6. Institute of Endemic Diseases, Xi’an Jiaotong University, College of Medicine, Xi’an, Shaanxi Province, 710061, China
  
• 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
• 出版者：Springer US
• ISSN：1559-0720

文摘

The objectives of this study are to assess T-2 toxin’s involvement in low selenium (Se)-induced Kashin-Beck disease (KBD) in rats and unveil the mechanisms underlying this disease. Two hundred thirty rats were randomly divided into two groups after weaning and fed normal or low-Se diets (n = 115), respectively, for a month. After low-Se model confirmation, rats in each group were subdivided into five: two subgroups (n = 20) were fed their current diets (normal or low-Se diets, respectively) for 30 and 90 days, respectively; two other subgroups (n = 25) received their current diets + low T-2 toxin (100 ng/g BW/day) for 30 and 90 days, respectively; and 25 rats were fed their current diets + high T-2 toxin (200 ng/g BW/day) for 30 days. Articular cartilage samples were extracted for hematoxylin and eosin (H&E) staining and immunohistochemistry. Western blot and reverse transcription-polymerase chain reaction (RT-PCR) were used to assess protein and mRNA levels, respectively, of collagen II, matrix metalloproteinase (MMP-1), MMP -3, MMP-13, and tissue inhibitor of metalloproteinase-1 (TIMP-1). Low Se and T-2 toxin synergistically affected animal fitness. Interestingly, low Se + T-2 toxin groups showed KBD characteristics. MMP-1, -3, and -13 mRNA and protein levels generally increased in low-Se groups, while collagen II and TIMP-1 levels showed a downward trend, compared with normal diet fed animals for the same treatment (P < 0.05). T-2 toxin’s effect was dose but not time dependent. Low Se and T-2 toxin synergistically alter the expression levels of collagen II as well as its regulatory enzymes MMP-1, MMP-3, MMP-13, and TIMP-1, inducing cartilage damage. Therefore, T-2 toxin may cause KBD in low-Se conditions. Keywords T-2 toxin Collagen II MMPs/TIMP-1 Kashin-Beck disease Rat model