Functional genomic analysis of Hawaii marine metagenomes

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• 作者：Xiaoqi Wang (1) (2)
  Qi Wang (1) (2)
  Xiao Guo (2)
  Luying Liu (1) (2)
  Jiangtao Guo (1) (2)
  Jinxian Yao (3)
  Huaiqiu Zhu (1) (2)
  
  1. Department of Biomedical Engineering ; College of Engineering ; Peking University ; Beijing ; 100871 ; China
  2. Center for Quantitative Biology ; Peking University ; Beijing ; 100871 ; China
  3. School of Life Sciences ; Peking University ; Beijing ; 100871 ; China
  
• 关键词：Marine microorganism ; Metagenome ; Gene annotation ; Codon usage bias ; Metabolic pathway ; 娴锋磱寰敓鐗?/li> 瀹忓熀鍥犵粍 ; 鍩哄洜鍔熻兘娉ㄩ噴 ; 瀵嗙爜瀛愬亸濂?/li> 浠ｈ阿閫氳矾
• 刊名：Chinese Science Bulletin
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：60
• 期：3
• 页码：348-355
• 全文大小：541 KB
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• 刊物主题：Science, general; Life Sciences, general; Physics, general; Chemistry/Food Science, general; Earth Sciences, general; Engineering, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-9541

文摘

Using high-throughput sequencing on metagenome to analyze marine microbial community, it is one of current main issues in the field of environmental microbe research. In this paper, we conducted the functional analysis on seven samples of metagenomic data from different depth seawater in Hawaii. The results of gene prediction and function annotation indicate that there are large amounts of potential novel genes of which functions remain unknown at present. Based on the gene annotation, codon usage bias is studied on ribosomal protein-related genes and shows an evident influence by the marine extreme environment. Furthermore, focusing on the marine environmental differences such as light intensity, dissolved oxygen, temperature and pressure among various depths, comparative analysis is carried out on related genes and metabolic pathways. Thus, the understanding as well as new insights into the correlation between marine environment and microbes are proposed at molecular level. Therefore, the studies herein afford a clue to reveal the special living strategies of microbial community from sea surface to deep sea.