Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells

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• 作者：Ying-hui Chen (1) (2)
  Lyh-Jyh Hao (3)
  Chih-peng Hung (4)
  Jung-wei Chen (5)
  Sew-fen Leu (6)
  Bu-miin Huang (7)
  
• 关键词：cisplatin ; cordycepin ; apoptosis ; OC3 ; synergistic
• 刊名：Chinese Journal of Integrative Medicine
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：20
• 期：8
• 页码：624-632
• 全文大小：884 KB
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• 作者单位：Ying-hui Chen (1) (2)
  Lyh-Jyh Hao (3)
  Chih-peng Hung (4)
  Jung-wei Chen (5)
  Sew-fen Leu (6)
  Bu-miin Huang (7)
  
  1. Department of Anesthesia, Chi-Mei Medical Center, Liouying, Tainan, Taiwan, China
  2. Department of Nursing, Min-Hwei College of Health Care Management, Tainan, Taiwan, China
  3. Division of Endocrinology and Metabolism, Kaohsiung Veteran General Hospital Tainan Branch, Tainan, Taiwan, China
  4. Department of Anesthesiology, Chang Gung Memorial Hospital and Kaohsiung Medical Center, Chang Gung University, Kaohsiung, Taiwan, China
  5. Institute of Hot Spring Industry, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, China
  6. Department of Food Science and Technology, College of Human Ecology, HungKuang University, Taichung, Taiwan, China
  7. Department of Cell Biology and Anatomy, and College of Medicine, National Cheng Kung University, Tainan, Taiwan, China
  
• ISSN：1993-0402

文摘

Objective To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. Methods The influences of cisplatin (2.5 μg/mL) and/or cordycepin treatment (10 or 100 μmol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Results Data demonstrated that co-administration of cisplatin (2.5 μg/mL) and cordycepin (10 or 100 μmol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P P PARP) as compared to cisplatin or cordycepin alone treatment (P Conclusion Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.