Alterations of Epithelial Permeability by Helicobacter and IL-1&bgr; In Vitro

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• 作者：Matysiak Budnik ; Tamara ; Thomas Collignon ; Agn&egrave ; s ; Mé ; graud ; Francis ; Heyman ; Martine
• 关键词：Helicobacter pylori ; permeability ; rebamipide ; Ussing chamber ; IL ; 1
• 刊名：Digestive Diseases and Sciences
• 出版年：2001
• 出版时间：2001
• 年：2001
• 卷：46
• 期：7
• 页码：1558-1566
• 全文大小：308 KB

文摘

Infection with Helicobacter increases the transcellular passage of macromolecules across the epithelium, and this effect can be prevented by a gastroprotective agent rebamipide. The aim was to gain insight into the mechanisms involved. The HT29-19A intestinal epithelial cells grown on microporous filters as monolayers were incubated in the presence or absence of rebamipide (1 or 2 mM) with: (1) suspension of a wild H. pylori strain, (2) IL-1&bgr; (0.5 ng/ml) + IFN-&ggr; (2 units/ml). After incubation, the monolayers were submitted to evaluation of apoptosis by using the apoptotic cell death detection ELISA kit and to assessment of epithelial permeability in Ussing chamber where the ionic conductance (G), fluxes of mannitol (J$\_Man$) and of horseradish peroxidase in both intact (J$\_HRPi$)- and degraded (J$\_D$) form, were measured. H. pylori increased the intact HRP fluxes across the barrier (J$\_HRPi$ = 17 ± 20 vs 97 ± 70 ng/hr/cm$^2$, P < 0.007), an effect prevented by rebamipide (J$\_HRPi$ = 33 ± 34 ng/hr/cm$^2$, P < 0.006). IL-1&bgr; increased the ionic conductance (G = 5.5 ± 1.0 and 21.0 ± 7.0 mS/cm$^2$, P < 0.006), the intact HRP fluxes (J$\_HRPi$ = 18 ± 15 and 476 ± 344 ng/hr/cm$^2$, P < 0.006), and the apoptotic index of the cells (AI = 1 ± 0 vs 3.7 ± 0.8), all effects prevented by rebamipide (G = 12 ± 4.9 mS/cm$^2$, J$\_HRPi$ = 79 ± 38, AI = 1.6 ± 0.6, P < 0.03 as compared to IL-&bgr;-treated cells). In basal conditions, rebamipide increased the integrity of the barrier (G = 7.5 ± 2.3 vs 6.0 ± 1.8 mS/cm$^2$ for controls, P < 0.007). In conclusion, H. pylori as well as IL-1&bgr;, may alter epithelial permeability and rebamipide may exert its protective effect on gastric mucosa by reinforcing the epithelial barrier in normal conditions and by counteracting the deleterious effect of Helicobacter pylori and IL-1&bgr; on macromolecular permeability.