Electrochemical immunoassay for the biomarker 8-hydroxy-2᾿deoxyguanosine using a glassy carbon electrode modified with chitosan and poly(indole-5-carboxylic acid)

详细信息    查看全文

• 作者：Deng Pan ; Qiang Zhou ; Shengzhong Rong ; Guangteng Zhang ; Yannan Zhang…
• 关键词：Oxidative DNA damage ; Electrochemical impedance spectroscopy ; Cyclic voltammetry ; Differential pulse voltammetry
• 刊名：Microchimica Acta
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：183
• 期：1
• 页码：361-368
• 全文大小：1,159 KB
• 参考文献：1.Rahimi R, Salehi Z, Saravani MF, Hoor ZMB, Darvishi S (2014) Is high-intensity resistance exercise-induced oxidative DNA damage related to OGG1 Ser326Cys polymorphism in athletes? Sport Sci Health 10:159–163CrossRef
  2.Kumar RG, Spurthi MK, Kumar KG, Sahu SK, Rani SH (2012) Endothelial nitric oxide synthase polymorphism G298T in association with oxidative DNA damage in coronary atherosclerosis. J Genet 91:349–352CrossRef
  3.Isobe C, Abe T, Terayama Y (2010) Levels of reduced and oxidized coenzyme Q-10 and 8-hydroxy-2′-deoxyguanosine in the CSF of patients with Alzheimer’s disease demonstrate that mitochondrial oxidative damage and/or oxidative DNA damage contributes to the neurodegenerative process. J Neurol 257:399–404CrossRef
  4.Bishnoi S, Goyal RN, Shim YB (2014) A novel nanogold–single wall carbon nanotube modified sensor for the electrochemical determination of 8-hydroxyguanine, a diabetes risk biomarker. Bioelectrochemistry 99:24–29CrossRef
  5.Priolli DG, Canelloi TP, Lopes CO, Júlio CMV, Martinez NP, Açari DP, Cardinalli LA, Ribeiro ML (2013) Oxidative DNA damage and β-catenin expression in colorectal cancer evolution. Int J Colorectal Dis 28:713–722CrossRef
  6.Singh B, Chatterjee A, Ronghe AM, Bhat NK, Bhat HK (2013) Antioxidant-mediated up-regulation of OGG1 via NRF2 induction is associated with inhibition of oxidative DNA damage in estrogen-induced breast cancer. BMC Cancer 13:253–262CrossRef
  7.Raza Y, Khan A, Farooqui A, Mubarak M, Facista A, Akhtar SS, Khan S, Kazi JL, Bernstein C, Kazmi SU (2014) Oxidative DNA damage as a potential early biomarker of helicobacter pylori associated carcinogenesis. Pathol Oncol Res 20:839–846CrossRef
  8.Nunomura A, Moreira PI, Castellani RJ, Lee HG, Zhu XW, Smith MA, Perry G (2012) Oxidative damage to RNA in aging and neurodegenerative disorders. Neurotox Res 22:231–248CrossRef
  9.Grindel A, Müllner E, Brath H, Jäger W, Henriksen T, Poulsen HE, Marko D, Wagner KH (2014) Influence of polyphenol-rich apple pomace extract on oxidative damage to DNA in type 2 diabetes mellitus individuals. Cancer Metab 2(Suppl 1):25–26CrossRef
  10.Kato D, Komoriya M, Nakamoto K, Kurita R, Hirono S, Niwa O (2011) Electrochemical determination of oxidative damaged DNA with high sensitivity and stability using a nanocarbon film. Anal Sci 27:703–707CrossRef
  11.Kantha SS, Wada S, Takeuchi M, Watabe S, Ochi H (1996) A sensitive method to screen for hydroxyl radical scavenging activity in natural food extracts using competitive inhibition ELISA for 8-hydroxy deoxyguanosine. Biotechnol Tech 10:933–936
  12.Guan YQ, Zhou GB, Ye JN (2014) Fast quantification of salivary 8-hydroxy-2′-deoxyguanosine as DNA damage biomarker using CE with electrochemical detection. Chromatographia 77:603–607CrossRef
  13.Ravanat JL, Guicherd P, Tuce Z, Cadet J (1999) Simultaneous determination of five oxidative DNA lesions in human urine. Chem Res Toxicol 12:802–808CrossRef
  14.Gupta RC, Arif JM (2001) An improved 32P-postlabeling assay for the sensitive detection of 8-oxodeoxyguanosine in tissue DNA. Chem Res Toxicol 14:951–957CrossRef
  15.Zhang TT, Zhao HM, Fan XF, Chen S, Quan X (2015) Electrochemiluminescence immunosensor for highly sensitive detection of 8-hydroxy-2′-deoxyguanosine based on carbon quantum dot coated Au/SiO2 core–shell nanoparticles. Talanta 131:379–385CrossRef
  16.Ma XM, Zhou WQ, Mo DZ, Wang ZP, Xu JK (2015) Capacitance comparison of poly(indole-5-carboxylic acid) in different electrolytes and its symmetrical supercapacitor in HClO4 aqueous electrolyte. Synth Met 203:98–106CrossRef
  17.Maciejewska J, Pisarek K, Bartosiewicz I, Krysinski P, Jackowska K, Biegunski AT (2011) Selective detection of dopamine on poly(indole-5-carboxylic acid)/tyrosinase electrode. Electrochim Acta 56:3700–3706CrossRef
  18.Narang J, Chauhan H, Rani P, Pundir CS (2013) Construction of an amperometric TG biosensor based on AuPPy nanocomposite and poly (indole-5-carboxylic acid) modified Au electrode. Biosyst Bioprocess Eng 36:425–432CrossRef
  19.Chauhan N, Narang J, Pundir CS (2011) Immobilization of rat brain acetylcholinesterase on ZnS and poly(indole-5-carboxylic acid) modified Au electrode for detection of organophosphorus insecticides. Biosens Bioelectron 29:82–88CrossRef
  20.Talbi H, Billaud D, Louarn G, Pron A (2001) In-situ spectroscopic investigations of the redox behavior of poly(indole-5-carboxylic-acid) modified electrodes in acidic aqueous solutions. Spectrochimica Acta Part A 57:423–433CrossRef
  21.Billaud D, Humbert B, Thevenot L, Thomas P, Talbi H (2003) Electrochemical properties and Fourier transform-infrared spectroscopic investigations of the redox behaviour of poly(indole-5-carboxylic acid) in LiClO 4 -acetonitrile solutions. Spectrochimica Acta Part A 59:163–168CrossRef
  22.Derkus B, Emregul KC, Mazi H, Emregul E, Yumak T, Sinag A (2014) Protein A immunosensor for the detection of immunoglobulin G by impedance spectroscopy. Biosyst Bioprocess Eng 3:965–976CrossRef
  23.Sivakkumar SR, Angulakshmi N, Saraswathi R (2005) Characterization of poly (indole-5-carboxylic acid) in aqueous rechargeable cells. J Appl Polym Sci 98:917–920CrossRef
  24.Yang SL, Lu ZZ, Luo SL, Liu CB, Tang YH (2013) Direct electrodeposition of a biocomposite consisting of reduced graphene oxide, chitosan and glucose oxidase on a glassy carbon electrode for direct sensing of glucose. Microchim Acta 180:127–135CrossRef
  25.Nie GM, Cai T, Zhang SS, Bao Q, Xu JK (2007) Electrodeposition of poly(indole-5-carboxylic acid) in boron trifluoride diethyl etherate containing additional diethyl ether. Electrochim Acta 52:7097–7106CrossRef
  26.Sheveleva V, Zemskova LA, Zheleznov SV, Voit AV, Barinov NN, Kuryavyi VG, Sergienko VL (2008) Correlation of electrochemical and structural characteristics of chitosan-carbon fibrous materials. Russ J Appl Chem 81:247–253CrossRef
  27.Li XM, Xia JP, Zhang SS (2008) Label-free detection of DNA hybridization based on poly(indole-5-carboxylic acid) conducting polymer. Anal Chim Acta 622:104–110CrossRef
  28.Jin WJ, Yang GJ, Wu LP (2011) Detecting 5-morpholino-3-amino-2-oxazolidone residue in food with label-free electrochemical impedimetric immunosensor. Food Control 22:1609–1616CrossRef
  29.Liu H, Wang YS, Wang JC, Xue LH, Zhou B, Zhao H, Liu SD, Tang X, Chen SH, Li MH, Cao JX (2014) A colorimetric aptasensor for the highly sensitive detection of 8-hydroxy-2′-deoxyguanosine based on G-quadruplex-hemin DNAzyme. Anal Biochem 458:4–10CrossRef
  30.Gutiérrez A, Gutiérrez S, Garcea G, Galicia L, Rivas GA (2011) Determinatiom of 8-hydroxy 2′-deoxyguanosine using electrodes modified with a dispersion of carbon nanotubes in polyethylenimine. Electroanalysis 23:1221–1228CrossRef
  31.Zhang Q, Wang YQ, Meng XY, Dhar R, Huang HD (2013) Triple-stranded DNA containing 8-Oxo-7,8-dihydro-2′-deoxyguanosine: implication in the design of selective aptamer sensors for 8-Oxo-7, 8-dihydroguanine. Anal Chem 85:201–207CrossRef
  32.Zhang SW, Zou CJ, Luo N, Weng QF, Cai LS, Wu CY, Xing J (2010) Determination of urinary 8-hydroxy-2′-deoxyguanosine by capillary electrophoresis with molecularly imprinted monolith in-tube solid phase microextraction. Chin Chem Lett 21:85–88CrossRef
  33.Sun X, Zhu Y, Wang XY (2011) Amperometric immunosensor based on a protein a/deposited gold nanocrystals modified electrode for carbofuran detection. Sensors 11:11679–11691CrossRef
  
• 作者单位：Deng Pan (1)
  Qiang Zhou (1)
  Shengzhong Rong (2)
  Guangteng Zhang (3)
  Yannan Zhang (4)
  Fenghai Liu (2)
  Miaojing Li (2)
  Dong Chang (5)
  Hongzhi Pan (1)
  
  1. Department of Nutrition and Food Hygiene, Public health school of Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin, Heilongjiang, 150081, China
  2. Department of epidemiology and health statistics, Public health school, Mudanjiang Medical College, Mudanjiang, Heilongjiang, 157011, China
  3. Department of Nutrition, The Third Central Hospital of Tianjin, Tianjin, 300170, China
  4. Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, 100069, China
  5. Clinical Laboratory, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Road, Nangang District, Harbin, Heilongjiang, 150001, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

We describe an electrochemical immunosensor for the determination of 8-hydroxy-2′-deoxyguanine (8-OHdG), which is a marker substance indicating oxidative DNA damage. Poly(indole-5-carboxylic acid) (PICA) and chitosan were immobilized on a glassy carbon electrode (PICA/CHI/GCE) via electrochemical polymerization and self-assembly. The use of PICA/CHI results in an increased effective surface area for immunoresponse and promotes electron transfer kinetics. The electrode surface was further modified by dropping protein A (PA) onto it in order to bind antibody Fc fragments. Next, anti-8-OHdG was covalently immobilized on the electrode. Finally, bovine serum albumin was applied to block any remaining active sites in order to prevent nonspecific adsorption. The modified electrode was characterized by electrochemical impedance spectroscopy, cyclic voltammetry and differential pulse voltammetry, respectively. Under optimized conditions, the electron transfer between 8-OHdG and the PICA/CHI/PA was quasi-reversible with a formal potential of 0.16 V vs Ag/AgCl. The peak currents of differential pulse voltammetry are linearly related to the 8-OH-dG concentration in the 0.1 to 10,000 ng · mL−1 range, with a detection limit of 30 pg · mL−1 (at an S/N ratio of 3). The immunosensor displays excellent reproducibility and stability and represents a sensitive method for the specific determination of 8-OHdG in human urine.