Porcine aminopeptidase N binds to F4+ enterotoxigenic Escherichia coli fimbriae

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• 作者：Pengpeng Xia ; Yiting Wang ; Congrui Zhu ; Yajie Zou ; Ying Yang…
• 刊名：Veterinary Research
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：47
• 期：1
• 全文大小：2,462 KB
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• 作者单位：Pengpeng Xia (1) (2)
  Yiting Wang (1) (2)
  Congrui Zhu (3)
  Yajie Zou (1) (2)
  Ying Yang (1) (2)
  Wei Liu (1) (2)
  Philip R. Hardwidge (4)
  Guoqiang Zhu (1) (2)
  
  1. College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China
  2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China
  3. College of Animal Medicine, Nanjing Agriculture University, Nanjing, 210095, China
  4. College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA
  
• 刊物主题：Veterinary Medicine;
• 出版者：BioMed Central
• ISSN：1297-9716

文摘

F4+ enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4+ ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4+ E. coli to intestinal epithelial cells. Pengpeng Xia and Yiting Wang contributed equally to this work