Identification of a mosquitocidal toxin from Bacillus thuringiensis using mass spectrometry

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• 作者：Wenfei Zhang (1)
  Jie Zhang (1)
  Neil Crickmore (2)
  Zhongqi Wu (1)
  Yiran Yang (1)
  Jiangzhao Qian (1)
  Hongping Wu (1)
  Ruiping Wang (1)
  Xuanjun Fang (3)
  
• 关键词：Cry toxin ; Cry4Cb ; Culex quinquefasciatus
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：30
• 期：12
• 页码：3273-3277
• 全文大小：309 KB
• 参考文献：1. Antal Z, Rascle C, Fevre M, Bruel C (2004) Single oligonucleotide nested PCR: a rapid method for the isolation of genes and their flanking regions from expressed sequence tags. Curr Genet 46(4):240-46 CrossRef
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• 作者单位：Wenfei Zhang (1)
  Jie Zhang (1)
  Neil Crickmore (2)
  Zhongqi Wu (1)
  Yiran Yang (1)
  Jiangzhao Qian (1)
  Hongping Wu (1)
  Ruiping Wang (1)
  Xuanjun Fang (3)
  
  1. Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou, Hainan, China
  2. School of Life Sciences, University of Sussex, Falmer, Brighton, UK
  3. Hainan Institute of Tropical Agricultural Resources (HITAR), Sanya, 572025, Hainan, China
  
• ISSN：1573-0972

文摘

The Bacillus thuringiensis strain S2160-1 has previously been identified as being highly toxic to mosquito larvae and a viable alternative to strains currently used commercially to control these insects. A PCR approach had identified the presence of four putative insecticidal toxin genes (cry30Ea, cry30?Ga, cry50Ba and cry54Ba) in this strain, but did not identify the genes that encoding three of the main crystal toxin proteins of size 140 and 130 and 30?kDa. In this study we used mass spectrometry to identify the 130?kDa toxin as a rare Cry4 toxin (Cry4Cb3). The gene encoding this toxin was cloned and expressed and the toxin shown to have mosquitocidal activity against Culex quinquefasciatus.