Putative Role of a Streptomyces coelicolor-Derived α-Mannosidase in Deglycosylation and Antibiotic Production

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• 作者：Thangamani Rajesh (1)
  Jong-Min Jeon (1)
  Eunjung Song (2)
  Hae-Min Park (2)
  Hyung Min Seo (1)
  Hyun-Joong Kim (1)
  Da-Hye Yi (1)
  Yong-Hyun Kim (1)
  Kwon-Young Choi (2)
  Yun-Gon Kim (3)
  Hyung-Yeon Park (4)
  Yoo Kyung Lee (5)
  Yung-Hun Yang (1) (6)
  
• 关键词：Streptomyces coelicolor ; α ; Mannosidase ; Deglycosylation ; Antibiotic production
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：172
• 期：3
• 页码：1639-1651
• 全文大小：426 KB
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• 作者单位：Thangamani Rajesh (1)
  Jong-Min Jeon (1)
  Eunjung Song (2)
  Hae-Min Park (2)
  Hyung Min Seo (1)
  Hyun-Joong Kim (1)
  Da-Hye Yi (1)
  Yong-Hyun Kim (1)
  Kwon-Young Choi (2)
  Yun-Gon Kim (3)
  Hyung-Yeon Park (4)
  Yoo Kyung Lee (5)
  Yung-Hun Yang (1) (6)
  
  1. Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, South Korea
  2. School of Chemical and Biological Engineering, Bioengineering Institute, and Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, Seoul, 151-742, South Korea
  3. Chemical Engineering, Soongsil University, 511 Sangdo-dong, Seoul, 156-743, Republic of Korea
  4. Bio-MAX Institute, Seoul National University, Gwanak-gu, Seoul, 151-742, South Korea
  5. Arctic Research Center, Korea Polar Research Institute, KIOST, 26 Songdomirae-ro, Incheon, 406-840, South Korea
  6. Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul, 143-701, South Korea
  
• ISSN：1559-0291

文摘

SCO0948 was found to be the single open reading frame annotated to encode an α-mannosidase (AM1) in Streptomyces coelicolor M145. To characterize the protein, we overexpressed SCO0948 in Escherichia coli BL21(DE3). Recombinant AM1, with a molecular weight of 110?kDa, exhibited α-mannosidase activity toward 4-nitrophenyl-α-d-mannopyranoside with a K m of 4.61?mM, a V max of 101.6?mM/min, and a specific activity of 47.96?U/mg. Treatment of ovalbumin, a glycoprotein, with AM1 resulted in partial deglycosylation, as assessed by glycostaining and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The S. coelicolor deletion mutant for SCO0948 failed to produce α-mannosidase activity, confirming AM1 as the only α-mannosidase in S. coelicolor M145. Interestingly, the deletion mutant and a complementation strain produced lower levels of the antibiotics actinorhodin and undecylprodigiosin in glucose minimal media. The results indicate that AM1 as an α-mannosidase influences deglycosylation and antibiotic production in S. coelicolor M145.