Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy
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- 作者：Feiyue Xing (1)
Jiongkun Wang (1)
Mingqian Hu (2)
Yu Yu (3) (4)
Guoliang Chen (1)
Jing Liu (5) - 关键词：dendritic cell ; nanostructure ; adhesion force ; comparison
- 刊名：Nanoscale Research Letters
- 出版年：2011
- 出版时间：December 2011
- 年：2011
- 卷：6
- 期：1
- 全文大小：646KB
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Jiongkun Wang (1)
Mingqian Hu (2)
Yu Yu (3) (4)
Guoliang Chen (1)
Jing Liu (5)
1. Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou, 510632, China
2. Department of Chemistry, Jinan University, Guangzhou, 510632, China
3. Department of Immunology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, 33612, USA
4. Department of Blood and Marrow Transplantation, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, 33612, USA
5. Department of Stomatology, Jinan University, Guangzhou, 510632, China - ISSN：1556-276X
文摘
A comparative study of immature and mature bone marrow-derived dendritic cells (BMDCs) was first performed through an atomic force microscope (AFM) to clarify differences of their nanostructure and adhesion force. AFM images revealed that the immature BMDCs treated by granulocyte macrophage-colony stimulating factor plus IL-4 mainly appeared round with smooth surface, whereas the mature BMDCs induced by lipopolysaccharide displayed an irregular shape with numerous pseudopodia or lamellapodia and ruffles on the cell membrane besides becoming larger, flatter, and longer. AFM quantitative analysis further showed that the surface roughness of the mature BMDCs greatly increased and that the adhesion force of them was fourfold more than that of the immature BMDCs. The nano-features of the mature BMDCs were supported by a high level of IL-12 produced from the mature BMDCs and high expression of MHC-II on the surface of them. These findings provide a new insight into the nanostructure of the immature and mature BMDCs.