Characterization of adipose tissue mesenchymal stromal cell subsets with distinct plastic adherence

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• 作者：Jung-Hyun Park ; Ki-Joo Kim ; Jong Won Rhie…
• 关键词：Mesenchymal stromal cell ; Adherence ; Subsets
• 刊名：Tissue Engineering and Regenerative Medicine
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：13
• 期：1
• 页码：39-46
• 全文大小：3,601 KB
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• 作者单位：Jung-Hyun Park (1)
  Ki-Joo Kim (2)
  Jong Won Rhie (2)
  Il-Hoan Oh (1)
  
  1. Department of Medical Life Science, Catholic High-Performance Cell Therapy Center, College of Medicine, The Catholic University of Korea, Seoul, Korea
  2. Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea
  
• 刊物主题：Biomedicine general; Biomedical Engineering; Cell Biology;
• 出版者：Springer Netherlands
• ISSN：2212-5469

文摘

Mesenchymal stromal cells (MSCs) established by in-vitro adherence culture have been widely utilized for various cell therapeutic trials, but potential heterogeneity that can be caused by preparation methods are poorly characterized. In this study, we show that at least two distinct subsets of MSCs with different adherence to plastic surface exist in human adipose tissue-derived stromal vascular fraction (SVF); while 69% of total colony forming units in SVF adhere to the surface before 3 hrs of plating, 13–17% of colonogenic cells adhered to the surface at later period of 15 hr to 1 week after plating. Of note, the late adherent MSCs exhibited higher self-renewal of colony forming cells and higher proliferating potential with comparable level of osteogenic or adipogenic differentiation potential to the early adherence subsets. Moreover, late adherent cells exhibited distinct pattern of paracrine secretome including higher level secretion of cytokines than the early adherent subsets. Taken together, these results suggest the possibility that distinct adherence properties of MSCs can be another parameter of clonal heterogeneity in the subpopulations of adipose tissue MSCs and that it can be an important factor for optimization of MSC preparation for cell therapeutic trials. Keywords Mesenchymal stromal cell Adherence Subsets