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作者单位:Min Jeong Kim (1) Yong Cheol Shin (1) Jong Ho Lee (1) Seung Won Jun (1) Chang-Seok Kim (1) Yunki Lee (2) Jong-Chul Park (3) Soo-Hong Lee (4) Ki Dong Park (2) Dong-Wook Han (1)
1. Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Korea 2. Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Korea 3. Department of Medical Engineering, Cellbiocontrol Laboratory, Yonsei University College of Medicine, Seoul, 03722, Korea 4. Department of Biomedical Science, CHA University, Gyeonggi-do, 11160, Korea
刊物类别:Biomaterials;
刊物主题:Biomaterials;
出版者:BioMed Central
ISSN:2055-7124
文摘
Background Hydrogels can serve as three-dimensional (3D) scaffolds for cell culture and be readily injected into the body. Recent advances in the image technology for 3D scaffolds like hydrogels have attracted considerable attention to overcome the drawbacks of ordinary imaging technologies such as optical and fluorescence microscopy. Multiphoton microscopy (MPM) is an effective method based on the excitation of two-photons. In the present study, C2C12 myoblasts differentiated in 3D gelatin hydroxyphenylpropionic acid (GHPA) hydrogels were imaged by using a custom-built multiphoton excitation fluorescence microscopy to compare the difference in the imaging capacity between conventional microscopy and MPM.