Combination treatment with fasudil and clioquinol produces synergistic anti-tumor effects in U87 glioblastoma cells by activating apoptosis and autophagy
详细信息 查看全文
- 作者：Mingliang He ; Ming Luo ; Qingyu Liu ; Jingkao Chen ; Kaishu Li…
- 关键词：Fasudil ; Clioquinol ; Synergistic effect ; Apoptosis ; Autophagy ; Glioblastoma
- 刊名：Journal of Neuro-Oncology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：127
- 期：2
- 页码：261-270
- 全文大小：3,123 KB
- 参考文献：1.Dolecek TA, Propp JM, Stroup NE, Kruchko C (2012) CBTRUS statistical report: primary brain and central nervous system tumors diagnosed in the United States in 2005-2009. Neuro Oncol 14(Suppl 5):v1–v49. doi:10.1093/neuonc/nos218 CrossRef PubMed PubMedCentral
2.Chong CR, Sullivan DJ Jr (2007) New uses for old drugs. Nature 448:645–646. doi:10.1038/448645a CrossRef PubMed
3.Jiang P, Mukthavaram R, Chao Y, Bharati IS, Fogal V, Pastorino S, Cong X, Nomura N, Gallagher M, Abbasi T, Vali S, Pingle SC, Makale M, Kesari S (2014) Novel anti-glioblastoma agents and therapeutic combinations identified from a collection of FDA approved drugs. J Transl Med 12:13. doi:10.1186/1479-5876-12-13 CrossRef PubMed PubMedCentral
4.Ehrenreiter K, Kern F, Velamoor V, Meissl K, Galabova-Kovacs G, Sibilia M, Baccarini M (2009) Raf-1 addiction in Ras-induced skin carcinogenesis. Cancer Cell 16:149–160. doi:10.1016/j.ccr.2009.06.008 CrossRef PubMed
5.Chen M, Liu A, Ouyang Y, Huang Y, Chao X, Pi R (2013) Fasudil and its analogs: a new powerful weapon in the long war against central nervous system disorders? Expert Opin Investig Drugs 22:537–550. doi:10.1517/13543784.2013.778242 CrossRef PubMed
6.Deng L, Li G, Li R, Liu Q, He Q, Zhang J (2010) Rho-kinase inhibitor, fasudil, suppresses glioblastoma cell line progression in vitro and in vivo. Cancer Biol Ther 9:875–884CrossRef PubMed
7.Yang X, Di J, Zhang Y, Zhang S, Lu J, Liu J, Shi W (2012) The Rho-kinase inhibitor inhibits proliferation and metastasis of small cell lung cancer. Biomed Pharmacother 66:221–227. doi:10.1016/j.biopha.2011.11.011 CrossRef PubMed
8.Ohta T, Takahashi T, Shibuya T, Amita M, Henmi N, Takahashi K, Kurachi H (2012) Inhibition of the Rho/ROCK pathway enhances the efficacy of cisplatin through the blockage of hypoxia-inducible factor-1alpha in human ovarian cancer cells. Cancer Biol Ther 13:25–33. doi:10.4161/cbt.13.1.18440 CrossRef PubMed
9.Iorio F, Isacchi A, di Bernardo D, Brunetti-Pierri N (2010) Identification of small molecules enhancing autophagic function from drug network analysis. Autophagy 6:1204–1205. doi:10.4161/auto.6.8.13551 CrossRef PubMed
10.Cahoon L (2009) The curious case of clioquinol. Nat Med 15:356–359. doi:10.1038/nm0409-356 CrossRef PubMed
11.Tateishi J (2000) Subacute myelo-optico-neuropathy: clioquinol intoxication in humans and animals. Neuropathology 20(Suppl):S20–S24CrossRef PubMed
12.Gilland O (1984) A neurological evaluation of purported cases of SMON in Sweden. Acta Neurol Scand Suppl 100:165–169PubMed
13.Ding WQ, Liu B, Vaught JL, Yamauchi H, Lind SE (2005) Anticancer activity of the antibiotic clioquinol. Cancer Res 65:3389–3395. doi:10.1158/0008-5472.can-04-3577 CrossRef PubMed
14.Fan C, Wang W, Zhao B, Zhang S, Miao J (2006) Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells. Bioorg Med Chem 14:3218–3222. doi:10.1016/j.bmc.2005.12.035 CrossRef PubMed
15.Park BC, Park SH, Paek SH, Park SY, Kwak MK, Choi HG, Yong CS, Yoo BK, Kim JA (2008) Chloroquine-induced nitric oxide increase and cell death is dependent on cellular GSH depletion in A172 human glioblastoma cells. Toxicol Lett 178:52–60. doi:10.1016/j.toxlet.2008.02.003 CrossRef PubMed
16.Yu H, Lou JR, Ding WQ (2010) Clioquinol independently targets NF-kappaB and lysosome pathways in human cancer cells. Anticancer Res 30:2087–2092PubMed
17.Du T, Filiz G, Caragounis A, Crouch PJ, White AR (2008) Clioquinol promotes cancer cell toxicity through tumor necrosis factor alpha release from macrophages. J Pharmacol Exp Ther 324:360–367. doi:10.1124/jpet.107.130377 CrossRef PubMed
18.Chen D, Cui QC, Yang H, Barrea RA, Sarkar FH, Sheng S, Yan B, Reddy GP, Dou QP (2007) Clioquinol, a therapeutic agent for Alzheimer’s disease, has proteasome-inhibitory, androgen receptor-suppressing, apoptosis-inducing, and antitumor activities in human prostate cancer cells and xenografts. Cancer Res 67:1636–1644. doi:10.1158/0008-5472.can-06-3546 CrossRef PubMed
19.Park MH, Lee SJ, Byun HR, Kim Y, Oh YJ, Koh JY, Hwang JJ (2011) Clioquinol induces autophagy in cultured astrocytes and neurons by acting as a zinc ionophore. Neurobiol Dis 42:242–251. doi:10.1016/j.nbd.2011.01.009 CrossRef PubMed
20.Cao B, Li J, Zhou X, Juan J, Han K, Zhang Z, Kong Y, Wang J, Mao X (2014) Clioquinol induces pro-death autophagy in leukemia and myeloma cells by disrupting the mTOR signaling pathway. Sci Rep 4:5749. doi:10.1038/srep05749 PubMed PubMedCentral
21.Jo GH, Bogler O, Chwae YJ, Yoo H, Lee SH, Park JB, Kim YJ, Kim JH, Gwak HS (2014) Radiation-induced autophagy contributes to cell death and induces apoptosis partly in malignant glioma cells. Cancer Res Treat. doi:10.4143/crt.2013.159
22.Chou TC (2006) Theoretical basis, experimental design, and computerized simulation of synergism and antagonism in drug combination studies. Pharmacol Rev 58:621–681. doi:10.1124/pr.58.3.10 CrossRef PubMed
23.Kundu S, Kim TH, Yoon JH, Shin HS, Lee J, Jung JH, Kim HS (2014) Viriditoxin regulates apoptosis and autophagy via mitotic catastrophe and microtubule formation in human prostate cancer cells. Int J Oncol 45:2331–2340. doi:10.3892/ijo.2014.2659 PubMed
24.Tsujimoto Y, Shimizu S (2005) Another way to die: autophagic programmed cell death. Cell Death Differ 12(Suppl 2):1528–1534. doi:10.1038/sj.cdd.4401777 CrossRef PubMed
25.Zhang X, Dong Y, Zeng X, Liang X, Li X, Tao W, Chen H, Jiang Y, Mei L, Feng SS (2014) The effect of autophagy inhibitors on drug delivery using biodegradable polymer nanoparticles in cancer treatment. Biomaterials 35:1932–1943. doi:10.1016/j.biomaterials.2013.10.034 CrossRef PubMed
26.Vlahopoulos S, Critselis E, Voutsas IF, Perez SA, Moschovi M, Baxevanis CN, Chrousos GP (2014) New use for old drugs? Prospective targets of chloroquines in cancer therapy. Curr Drug Targets 15:843–851CrossRef PubMed
27.Jose P, Sundar K, Anjali CH, Ravindran A (2014) Metformin-loaded BSA nanoparticles in cancer therapy: a new perspective for an old antidiabetic drug. Cell Biochem Biophys. doi:10.1007/s12013-014-0242-8 PubMed
28.Li Y, Li PK, Roberts MJ, Arend RC, Samant RS, Buchsbaum DJ (2014) Multi-targeted therapy of cancer by niclosamide: a new application for an old drug. Cancer Lett 349:8–14. doi:10.1016/j.canlet.2014.04.003 CrossRef PubMed PubMedCentral
29.Burz C, Berindan-Neagoe I, Balacescu O, Irimie A (2009) Apoptosis in cancer: key molecular signaling pathways and therapy targets. Acta Oncol 48:811–821. doi:10.1080/02841860902974175 CrossRef PubMed
30.Ghobrial IM, Witzig TE, Adjei AA (2005) Targeting apoptosis pathways in cancer therapy. CA 55:178–194PubMed
31.Schmitt CA (2003) Senescence, apoptosis and therapy–cutting the lifelines of cancer. Nat Rev Cancer 3:286–295. doi:10.1038/nrc1044 CrossRef PubMed
32.Taylor RC, Cullen SP, Martin SJ (2008) Apoptosis: controlled demolition at the cellular level. Nat Rev Mol Cell Biol 9:231–241. doi:10.1038/nrm2312 CrossRef PubMed
33.Cao B, Li J, Zhu J, Shen M, Han K, Zhang Z, Yu Y, Wang Y, Wu D, Chen S, Sun A, Tang X, Zhao Y, Qiao C, Hou T, Mao X (2013) The antiparasitic clioquinol induces apoptosis in leukemia and myeloma cells by inhibiting histone deacetylase activity. J Biol Chem 288:34181–34189. doi:10.1074/jbc.M113.472563 CrossRef PubMed PubMedCentral
34.Takeba Y, Matsumoto N, Watanabe M, Takenoshita-Nakaya S, Ohta Y, Kumai T, Takagi M, Koizumi S, Asakura T, Otsubo T (2012) The Rho kinase inhibitor fasudil is involved in p53-mediated apoptosis in human hepatocellular carcinoma cells. Cancer Chemother Pharmacol 69:1545–1555. doi:10.1007/s00280-012-1862-6 CrossRef PubMed
35.Su M, Mei Y, Sinha S (2013) Role of the crosstalk between autophagy and apoptosis in cancer. J Oncol 2013:102735. doi:10.1155/2013/102735 CrossRef PubMed PubMedCentral
36.Yu L, Alva A, Su H, Dutt P, Freundt E, Welsh S, Baehrecke EH, Lenardo MJ (2004) Regulation of an ATG7-beclin 1 program of autophagic cell death by caspase-8. Science 304:1500–1502. doi:10.1126/science.1096645 CrossRef PubMed
37.Santoni M, Amantini C, Morelli MB, Liberati S, Farfariello V, Nabissi M, Bonfili L, Eleuteri AM, Mozzicafreddo M, Burattini L, Berardi R, Cascinu S, Santoni G (2013) Pazopanib and sunitinib trigger autophagic and non-autophagic death of bladder tumour cells. Br J Cancer 109:1040–1050. doi:10.1038/bjc.2013.420 CrossRef PubMed PubMedCentral
38.Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T (2011) Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells. Acta Pharmacol Sin 32:1266–1275. doi:10.1038/aps.2011.92 CrossRef PubMed PubMedCentral - 作者单位：Mingliang He (1)
Ming Luo (2)
Qingyu Liu (3)
Jingkao Chen (4)
Kaishu Li (1)
Meiguang Zheng (1)
Yinlun Weng (1)
Leping Ouyang (1)
Anmin Liu (1)
1. Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, People’s Republic of China
2. Department of Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, People’s Republic of China
3. Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, People’s Republic of China
4. School of Pharmaceutical Sciecnes, Sun Yat-Sen University, Guangzhou, 510006, China - 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Oncology - 出版者：Springer Netherlands
- ISSN：1573-7373
文摘
Survival of patients with glioblastoma (GBM) remains poor, and novel treatment methods are urgently needed. In this study, we tested the effects of a combination of fasudil, a ROCK inhibitor, and clioquinol, an 8-hydroxyquinoline derivative with antimicrobial properties, on human GBM U87 cells. Combination treatment synergistically inhibited the viability of glioma cells but not mouse normal neuron HT22 cells and significantly induced mitochondria-mediated apoptosis. Moreover, the combination was also found to trigger macro-autophagy (henceforth referred to as autophagy) by increasing the expression levels of several proteins involved in the induction of autophagy. Further studies showed that 3-methyladenine (3-MA) or chloroquine (CQ), two autophagy inhibitors, abrogated the cytotoxic effects of the combination treatment as well as the autophagy. Overall, we demonstrated that fasudil and clioquinol show synergistic anti-cancer effects, providing evidence for the further development of combination therapy for GBM.