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The interface layer and discrete crystal growth in solidification are traced by analyzing the microsegregation of aluminum alloy. Based on the interface evolution and discrete crystal growth, the non-equilibrium microsegregation mechanism is qualitatively proposed. The solute content of solid is decided by the solidification temperature of interface. And the solidification temperature of interface is further decided by the evolution of interface compositions and interface temperature. The fluctuation of interface compositions and actual interface temperature persists in the whole solidification process.