Molecular cloning, expression pattern, and phylogenetic analysis of a tetraspanin CD82-like molecule in lamprey Lampetra japonica

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• 作者：Xiaoping Zhang ; Xueying Song ; Peng Su ; Meng Gou…
• 关键词：CD82 ; Lampetra japonica ; Expression pattern ; Molecular evolution ; Immune response
• 刊名：Development Genes and Evolution
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：226
• 期：2
• 页码：87-98
• 全文大小：792 KB
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• 作者单位：Xiaoping Zhang (1) (2)
  Xueying Song (1) (2)
  Peng Su (1) (2)
  Meng Gou (1) (2)
  Hao Wang (1) (2)
  Xin Liu (1) (2)
  Qingwei Li (1) (2)
  
  1. College of Life Science, Liaoning Normal University, Dalian, 116029, China
  2. Lamprey Research Center, Liaoning Normal University, Dalian, 116029, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Developmental Biology
  Neurosciences
  Cell Biology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-041X

文摘

CD82, a member of the tetraspanins, is originally identified as an accessory molecule in T cell activation, and it participates in the formation of immune synapse both in T cells and antigen-presenting cells of jawed vertebrates. In the present study, a CD82 homologous complementary DNA (cDNA) sequence is identified in the lamprey Lampetra japonica. The open reading frame of this sequence is 801 bp long and encodes a 266-amino acid protein. The multialignment of this sequence with several typical CD82s and CD37s of jawed vertebrates shows that it also possesses their conserved four transmembrane domains and a six-cysteine motif Cys-Cys-Gly…Cys-Ser-Cys…Cys…Cys, which is a characteristic motif of CD82 and CD37 vertebrate tetraspanin sequences. Since it is close to CD82s in sequence similarity, we name it as Lja-CD82-like. From the distribution profile of the conserved motifs of CD82-like, CD82, and CD37 molecules from molluscas to mammals, it seems that the CD82s and CD37s evolved from a common ancestral gene through a gene duplication event to their modern forms by a short insertion or substitution approaches. The phylogenetic analysis indicated that CD82 and CD37 molecules of jawed vertebrates originated from a common ancestral gene which is close to agnathan CD82-like and evolved into two distinct paralogous groups maybe after the divergence of jawed and jawless vertebrates. An expression vector with trigger factor (TF) was constructed to ensure that Lja-CD82-like express in prokaryotic expression host. The expressions of Lja-CD82-like messenger RNA (mRNA) and protein in immune-related tissues of lamprey were detected by real-time quantitative polymerase chain reaction and western blotting. Results showed that the mRNA and the protein levels of Lja-CD82-like were significantly upregulated in lymphocyte-like cells, gills, and supraneural myeloid bodies after stimulation with mixed antigens, respectively. Our data provided a foundation for the further study of Lja-CD82-like and its role in immune response process of jawless vertebrates.