The tyrosyl-DNA phosphodiesterase gene family in Medicago truncatula Gaertn.: bioinformatic investigation and expression profiles in response to copper- and PEG-mediated stress
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- 作者：Anca Macovei (1)
Alma Balestrazzi (1)
Massimo Confalonieri (2)
Daniela Carbonera (1) - 关键词：Barrel medic ; DNA topoisomerase I ; Oxidative stress ; Seed ; Tdp1 gene ; top1 gene ; Tyrosyl ; DNA phosphodiesterase
- 刊名：Planta
- 出版年：2010
- 出版时间：July 2010
- 年：2010
- 卷：232
- 期：2
- 页码：393-407
- 全文大小：856KB
- 参考文献：1. Armengaud P, Thiery L, Buhot N, Grenier-de March G, Savourè A (2004) Transcriptional regulation of proline biosynthesis in / Medicago truncatula reveals developmental and environmental specific features. Physiol Plant 120:442-50 CrossRef
2. Bailly C (2004) Active oxygen species and antioxidants in seed biology. Seed Sci Res 14:93-07 CrossRef
3. Balestrazzi A, Chini A, Bernacchia G, Bracci A, Luccarini G, Cella R, Carbonera D (2000) Carrot cells contain two / top1 genes having the coding capacity for two distinct DNA topoisomerase I. J Exp Bot 51:1979-990 CrossRef
4. Balestrazzi A, Botti S, Zelasco S, Biondi S, Franchin C, Calligari P, Racchi M, Turchi A, Lingua G, Berta G, Carbonera D (2009) Expression of the / PsMT / A1 gene in white poplar engineered with the MAT system is associated to heavy metal tolerance and protection against 8-hydroxy-2-deoxyguanosine mediated-DNA damage. Plant Cell Rep 28:1179-192 CrossRef
5. Balestrazzi A, Locato V, Bottone MG, De Gara L, Biggiogera M, Pellicciari C, Botti S, Di Gesù D, Donà M, Carbonera D (2010) Response to UV-C radiation in topo I-deficient carrot cells with low ascorbate levels. J Exp Bot 61:575-85 CrossRef
6. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 270:343-53
7. Brameier M, Krings A, MacCallum RM (2007) NucPred-predicting nuclear localization of proteins. Bioinformatics 23:1159-160 CrossRef
8. Brunner AM, Jakovlev IA, Strauss SH (2004) Validating internal controls for quantitative plant gene expression studies. BMC Plant Biol 4:14 CrossRef
9. Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169-93 CrossRef
10. de Castro RD, van Lammeren AAM, Groot SPC, Bino RJ, Hilhorst HWM (2000) Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not. Plant Physiol 122:327-35 CrossRef
11. El-Khamisy SF, Saifi GM, Weinfeld M, Johansson F, Helleday T, Lupski JR, Caldecott KW (2005) Defective DNA single-strand break repair in spinocerebellar ataxia with axonal neuropathy. Nature 434:108-13 CrossRef
12. El-Tayeb MA, El-Enany AE, Ahmed NL (2006) Salycilic acid-induced adaptive response to copper stress in sunflower ( / Helianthus annuus L.). Plant Growth Regul 50:191-99 CrossRef
13. Fryer MJ, Oxborough K, Mullineaux PM, Baker NR (2002) Imaging of photooxidative stress responses in leaves. J Exp Bot 53:1249-254 CrossRef
14. Gengsheng X, Kairong C, Ji L, Yafu W, Zhixiao L (2000) Water stress and accumulation of β-N-oxalyl-L-α, β-diaminopropionic acid in grass pea ( / Lathyrus sativus). J Agric Food Chem 49:216-20
15. Hettiarachchi GH, Reddy MK, Sopory SK, Chattopadhyay S (2005) Regulation of TOP2 by various abiotic stresses including cold and salinity in pea and transgenic tobacco plants. Plant Cell Physiol 46:1154-160 CrossRef
16. Inamdar KV, Pouliot JJ, Zhou T, Lees-Miller SP, Rasouli-Nia A, Povirk LF (2002) Conversion of phosphoglycolate to phosphate termini on 3-overhangs of DNA double strand breaks by the human tyrosyl-DNA phosphodiesterase hTdp1. J Biol Chem 277:27162-7168 CrossRef
17. Interthal H, Pouliot JJ, Champoux JJ (2001) The tyrosyl-DNA phosphodiesterase Tdp1 is a member of the phospholipase D superfamily. Proc Natl Acad Sci USA 98:12009-2014 CrossRef
18. Ishikawa T, Shigeoka S (2008) Recent advances in ascorbate biosynthesis and the physiological significance of ascorbate peroxidase in photosynthesizing organisms. Biosci Biotech Biochem 72:1143-154 CrossRef
19. Jonak C, Nakagami H, Hirt H (2004) Heavy metal stress. Activation of distinct mitogen-activated protein kinase pathways by copper and cadmium. Plant Physiol 136:3276-283 CrossRef
20. Kimura S, Sakaguchi K (2006) DNA repair in plants. Chem Rev 106:753-66 CrossRef
21. Lebedeva N, Rechkunova N, Boiteux S, Lavrik O (2008) Trapping of human DNA topoisomerase I by DNA structures mimicking intermediates of DNA repair. IUBMB Life 60:130-34 CrossRef
22. Lima WC, Medina-Silva R, Galhardo RS, Menck CFM (2001) Distribution of DNA repair-related ESTs in sugarcane. Gen Mol Biol 24:141-46
23. Locato V, Balestrazzi A, De Gara L, Carbonera D (2006) Reduced expression of / top1β gene induces programmed cell death and alters ascorbate metabolism in / Daucus carota cultured cell. J Exp Bot 57:1667-676 CrossRef
24. Lu T, Pan Y, Kao SY, Li C, Kohane I, Chan J, Yankner BA (2004) Gene regulation and DNA damage in the ageing human brain. Nature 429:883-91 CrossRef
25. Luo Y, Liu YB, Dong YX, Gao X-Q, Zhang XS (2009) Expression of a putative alfalfa helicase increases tolerance to abiotic stress in / Arabidopsis by enhancing the capacities for ROS scavenging and osmotic adjustments. J Plant Physiol 166:385-94 CrossRef
26. Mielke C, Kalfalah FM, Christensen MO, Boege F (2007) Rapid and prolonged stalling of human DNA topoisomerase I in UVA-irradiated genomic areas. DNA Repair 6:1757-763 CrossRef
27. Miura K, Jin JB, Hasegawa PM (2007) Sumoylation, a post translational regulatory process in plants. Curr Opin Plant Biol 10:495-02 CrossRef
28. Murali Achary VM, Panda BB (2009) Aluminium-induced DNA damage and adaptive response to genotoxic stress in plant cells are mediated through reactive oxygen intermediates. Mutagenesis 25:201-09 CrossRef
29. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:73-9 CrossRef
30. Nunes C, de Sousa-Araujo S, Marques da Silva J, Fevereiro MPS, Bernardes da Silva A (2008) Physiological responses of the legume model / Medicago truncatula cv Jemalong to water deficit. Environ Exp Bot 63:289-96 CrossRef
31. Oh M-N, Trick HN, Rajashekar CB (2008) Secondary metabolism and antioxidants are involved in environmental adaptation and stress tolerance in lettuce. J Plant Physiol 166:180-91 CrossRef
32. Pommier Y, Redon C, Rao VA, Seiler JA, Sordet O, Takemura H, Antony S, Meng L, Liao Z, Kohlhagen G, Zhang H, Kohn KW (2003) Repair of and checkpoint response to topoisomerase I-mediated DNA damage. Mutat Res 532:173-03
33. Prasad MNV, Malec P, Waloszek A (2001) Physical responses of / Lemna trisulca L. to cadmium and copper bioaccumulation. Plant Sci 161:881-89 CrossRef
34. Ramakers C, Ruijter JM, Lekanne Deprez RH, Moorman AFM (2003) Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci Lett 339:62-6 CrossRef
35. Rogers SO, Bendich AJ (1989) Extraction of DNA from plant tissues. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual. Kluwer Academic Publishers, Dordrecht, pp 1-0
36. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
37. Sanan-Mishra N, Pham XH, Sopory SK, Tuteja N (2005) Pea DNA helicase 45 overexpression in tobacco confers high salinity tolerance without affecting yield. Proc Natl Acad Sci USA 102:509-14 CrossRef
38. Schenk RV, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199-04 CrossRef
39. Schoeffler AJ, Berger JM (2008) DNA topoisomerases: harnessing and constraining energy to govern chromosome topology. Q Rev Biophys 41:41-01
40. Schutzendubel A, Polle A (2002) Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot 53:1351-365 CrossRef
41. Singh BN, Sopory SK, Reddy MK (2004) Plant DNA topoisomerases: structure, function and cellular roles in plant development. Crit Rev Plant Sci 23:251-69 CrossRef
42. Takagi H (2008) Proline as a stress protectant in yeast: physiological functions, metabolic regulations, and biotechnological applications. Appl Microb Biotech 81:211-23 CrossRef
43. Takahashi T, Matsuhara S, Abe M, Komeda Y (2002) Disruption of a DNA topoisomerase I gene affects morphogenesis in Arabidopsis. Plant Cell 14:2085-093 CrossRef
44. Takashima H, Boerkoel CF, John J, Saifi GM, Salih MA, Armostrong D, Mao Y, Quiocho FA, Roa BB, Nakagawa M, Stockton DW, Lupski JR (2002) Mutation of TDP1, encoding a topoisomerase I-dependent DNA damage repair enzyme, in spinocerebellar ataxia with axonal neuropathy. Nat Genet 32:267-72 CrossRef
45. Thordal-Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley—powdery mildew interaction. Plant J 11:1187-194 CrossRef
46. Trinh TH, Ratet P, Kondorosi E, Durand P, Kamatè K, Bauer P, Kondorosi A (1998) Rapid and efficient transformation of diploid / Medicago truncatula and / Medicago sativa ssp. falcata lines improved in somatic embryogenesis. Plant Cell Rep 17:345-55 CrossRef
47. Wang F-Z, Wang Q-B, Kwon S-Y, Kwak S-S, Su W-A (2005) Enhanced drought tolerance of transgenic rice plants expressing a pea manganese superoxide dismutase. J Plant Physiol 162:465-72 CrossRef
48. Wellburn AR (1994) The spectral determination of chlorophylls / a and / b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144:307-13
49. Whittle C-A, Beardmore T, Johnston MO (2001) Is G1 arrest in plant seeds induced by a p53-related pathway? Trends Plant Sci 6:248-51 CrossRef
50. Yang SW, Burgin AB Jr, Huizenga BN, Robertson CA, Yao KC, Nash HA (1996) A eukaryotic enzyme that can disjoin dead-end covalent complexes between DNA and type I topoisomerases. Proc Natl Acad Sci USA 93:11534-1539 CrossRef
51. Yoshimura K, Yabuta Y, Ishikawa T, Shigeoka S (2000) Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stress. Plant Physiol 123:223-33 CrossRef
52. Yurchenko V, Xue Z, Sadofsky MJ (2006) SUMO modification of human XRCC4 regulates its localization and function in DNA double-strand break repair. Mol Cell Biol 26:1786-794 CrossRef - 作者单位：Anca Macovei (1)
Alma Balestrazzi (1)
Massimo Confalonieri (2)
Daniela Carbonera (1)
1. Dipartimento di Genetica e Microbiologia, Via Ferrata 1, 27100, Pavia, Italy
2. C.R.A. Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Viale Piacenza 29, 26900, Lodi, Italy
文摘
The Tdp1 gene encoding tyrosyl-DNA phosphodiesterase has been extensively investigated in animal cells, due to the role of this enzyme in the repair of topoisomerase I-DNA covalent lesions. In contrast, information in this regard is totally missing in plants. We report for the first time in plants on the Tdp1 gene family from barrel medic (Medicago truncatula Gaertn.), composed of two members, hereby named MtTdp1α and MtTdp1β. The expression profiles of MtTdp1α and MtTdp1β genes were evaluated in plantlets grown in vitro using copper and polyethylene glycol (PEG 6000) as stress agents. In situ detection of reactive oxygen species (ROS) was carried out by histochemical staining, while the level of oxidative DNA damage, quantified in terms of 7,8-dihydro-8-oxoguanine (8-oxo-dG), increased up to 7.4- and 6.7-fold in response to copper and PEG 6000 treatments, respectively. Quantitative real-time polymerase chain reaction revealed that both Tdp1 genes were significantly up-regulated in response to copper and PEG. The Tdp1 genes were also significantly up-regulated during seed rehydration, an aspect of seed physiology in which DNA repair is a key component. Thus, the Tdp1 genes might be used as novel tools for improving stress tolerance in crops. The expression patterns of the barrel medic top1α and top1β genes, encoding distinct isoforms of DNA topoisomerase I, were also analyzed and discussed to acquire additional information on their specific functions, closely related to that of the Tdp1 gene in animal cells.