Upregulation of long non-coding RNA MALAT1 correlates with tumor progression and poor prognosis in clear cell renal cell carcinoma

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• 作者：Hai-min Zhang ; Feng-qiang Yang ; Shao-Jun Chen ; Jianping Che ; Jun-hua Zheng
• 关键词：MALAT1 ; Clear cell renal cell carcinoma ; Proliferation ; Invasion ; Migration
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：36
• 期：4
• 页码：2947-2955
• 全文大小：949 KB
• 参考文献：1.Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013. CA Cancer J Clin. 2013;63(1):11鈥?0.View Article PubMed
  2.Yang FQ, Yang FP, Li W, et al. Foxl1 inhibits tumor invasion and predicts outcome in human renal cancer. Int J Clin Exp Pathol. 2014;7(1):110.PubMed Central PubMed
  3.Russo P. Renal cell carcinoma: presentation, staging, and surgical treatment. Semin Oncol. 2000;27(2):160鈥?6.PubMed
  4.Motzer RJ, Molina AM. Targeting renal cell carcinoma. J Clin Oncol. 2009;27(20):3274鈥?.View Article PubMed
  5.Prensner JR, Chinnaiyan AM. The emergence of lncRNAs in cancer biology. Cancer Discov. 2011;1(5):391鈥?07.View Article PubMed Central PubMed
  6.Esteller M. Non-coding RNAs in human disease. Nat Rev Genet. 2011;12:861鈥?4.View Article PubMed
  7.Gibb EA, Brown CJ, Lam WL. The functional role of long non-coding RNA in human carcinomas. Mol Cancer. 2011;10:38.View Article PubMed Central PubMed
  8.Wang P, Ren Z, Sun P. Overexpression of the long non-coding RNA MEG3 impairs in vitro glioma cell proliferation. J Cell Biochem. 2012;113:1868鈥?4.View Article PubMed
  9.Gupta RA, Shah N, Wang KC, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 2010;464(7291):1071鈥?.View Article PubMed Central PubMed
  10.Han Y, Liu Y, Gui Y, et al. Long intergenic non-coding RNA TUG1 is overexpressed in urothelial carcinoma of the bladder. J Surg Oncol. 2013;107(5):555鈥?.View Article PubMed
  11.Xie H W, Wu Q Q, Zhu B, et al. Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis. Tumor Biology. 2014;1鈥?2.
  12.Tripathi V, Ellis JD, Shen Z, et al. The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation. Mol Cell. 2010;39(6):925鈥?8.View Article PubMed Central PubMed
  13.Ji P, Diederichs S, Wang W, et al. MALAT-1, a novel noncoding RNA, and thymosin 尾4 predict metastasis and survival in early-stage non-small cell lung cancer. Oncogene. 2003;22(39):8031鈥?1.View Article PubMed
  14.Lai M, Yang Z, Zhou L, et al. Long non-coding RNA MALAT-1 overexpression predicts tumor recurrence of hepatocellular carcinoma after liver transplantation. Med Oncol. 2012;29(3):1810鈥?.View Article PubMed
  15.Liu JH, Chen G, Dang YW, et al. Expression and prognostic significance of lncRNA MALAT1 in pancreatic cancer tissues. Asian Pac J Cancer Prev. 2014;15(7):2971.View Article PubMed
  16.Gutschner T, H盲mmerle M, Ei脽mann M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res. 2013;73(3):1180鈥?.View Article PubMed Central PubMed
  17.Yang L, Lin C, Liu W, et al. ncRNA-and Pc2 methylation-dependent gene relocation between nuclear structures mediates gene activation programs. Cell. 2011;147(4):773鈥?8.View Article PubMed Central PubMed
  18.Wu XS, Wang XA, Wu WG, et al. MALAT1 promotes the proliferation and metastasis of gallbladder cancer cells by activating the ERK/MAPK pathway. Cancer Biol Ther. 2014;15(6):0鈥?.
  19.Tee AE, Ling D, Nelson C, et al. The histone demethylase JMJD1A induces cell migration and invasion by up-regulating the expression of the long noncoding RNA MALAT1. Oncotarget. 2014;5(7):1793鈥?04.PubMed Central PubMed
  20.Carninci P, Kasukawa T, Katayama S, et al. The transcriptional landscape of the mammalian genome. Science. 2005;309(5740):1559鈥?3.View Article PubMed
  21.Esteller M. Non-coding RNAs in human disease. Nat Rev Genet. 2011;12(12):861鈥?4.View Article PubMed
  22.Gutschner T, H盲mmerle M, Diederichs S. MALAT1鈥攁 paradigm for long noncoding RNA function in cancer. J Mol Med. 2013;91(7):791鈥?01.View Article PubMed
  23.Guo F, Li Y, Liu Y, et al. Inhibition of metastasis-associated lung adenocarcinoma transcript 1 in CaSki human cervical cancer cells suppresses cell proliferation and invasion. Acta Biochim Biophys Sin. 2010;42(3):224鈥?.View Article PubMed
  
• 作者单位：Hai-min Zhang (1)
  Feng-qiang Yang (1)
  Shao-Jun Chen (1)
  Jianping Che (1)
  Jun-hua Zheng (1)
  
  1. Department of Urology, Shanghai Tenth People鈥檚 Hospital, Tongji University, No 301, Yanchang Mid-Road, Shanghai, 200072, China
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

Long noncoding RNAs (lncRNAs) have been investigated as a new class of regulators of cellular processes, such as cell growth, apoptosis, and carcinogenesis. LncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has recently been identified to be involved in tumorigenesis of several cancers such as lung cancer, pancreatic cancer, and cervical cancer. However, the role of lncRNA MALAT1 in clear cell renal cell carcinoma (ccRCC) remains unclear. Expression levels of lncRNA MALAT1 in ccRCC tissues and renal cancer cell lines were evaluated by quantitative real-time PCR (qRT-PCR), and its association with overall survival of patients was analyzed by statistical analysis. Small interfering RNA (siRNA) was used to suppress MALAT1 expression in renal cancer cells. In vitro assays were conducted to further explore its role in tumor progression. The expression level of MALAT1 was higher in ccRCC tissues and renal cancer cells compared to adjacent non-tumor tissues and normal human proximal tubule epithelial cells HK-2. The ccRCC patients with higher MALAT1 expression had an advanced clinical features and a shorter overall survival time than those with lower MALAT1 expression. And multivariate analysis showed that the status of MALAT1 expression was an independent predictor of overall survival in ccRCC. Additionally, our data indicated that knockdown expression of MALAT1 decreased renal cancer cell proliferation, migration, and invasion. Our data suggested that lncRNA MALAT1 was a novel molecule involved in ccRCC progression, which provided a potential prognostic biomarker and therapeutic target.