Effects of the exposure to atrazine on bone development of Podocnemis expansa (Testudines, Podocnemididae)

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• 作者：Juliana dos Santos Mendonça ; Lucélia Gonçalves Vieira…
• 关键词：Pesticides ; Contamination ; Ecotoxicology ; Skeleton ; Reptiles ; Giant Amazon river turtle
• 刊名：Ecotoxicology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：25
• 期：3
• 页码：594-600
• 全文大小：866 KB
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• 作者单位：Juliana dos Santos Mendonça (1)
  Lucélia Gonçalves Vieira (1)
  Sady Alexis Chavauty Valdes (1)
  Franz Zirena Vilca (2)
  Valdemar Luiz Tornisielo (2)
  André Luiz Quagliatto Santos (1)
  
  1. Wild Animal Research and Teaching Laboratory (LAPAS), Universidade Federal de Uberlândia (UFU), Rua Piaui, S/N, Campos Umuarama, Av. Jardim Umuarama, Uberlândia, MG, CEP 38400-902, Brazil
  2. Ecotoxicology Laboratory, Centro de Energia Nuclear na Agricultura da Universidade de São Paulo (CENA/USP), Piracicaba, SP, Brazil
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  Ecology
  Environmental Management
  
• 出版者：Springer Netherlands
• ISSN：1573-3017

文摘

The use of pesticides is a widely spread practice in Brazilian agriculture, and dispersion of these substances is an important factor for the fauna and flora. Atrazine is an endocrine disruptor in the xenoestrogen class that is used worldwide in agricultural practices. In Brazil, its use is permitted in several crops. Podocnemis expansa is a representative of the Testudines order that is the largest freshwater reptile of South America. Its distribution enables it to get in contact with molecules that are commonly used as pesticides, which may cause deleterious effects in target populations. In order to evaluate the possible effects of the exposure to atrazine on bone ontogeny of this species, eggs were artificially incubated in sand moistened with water contaminated with atrazine at concentrations equal to 0, 2, 20 or 200 μg/L. Embryos were collected throughout incubation and submitted to diaphanization of soft tissues with potassium hydroxide (KOH); bones were stained with Alizarin red S and cartilages by Alcian blue. Embryos were evaluated for the presence of abnormalities during the different stages of pre-natal development of skeletal elements. No effect of atrazine was observed on bone development during the embryonic phase in P. expansa individuals, in the conditions of this study.