Genome-Wide Identification, Expression, and Activity Analysis of Alkaline/Neutral Invertase Gene Family from Cassava (Manihot esculenta Crantz)
详细信息 查看全文
- 作者：Yuan Yao ; Meng-Ting Geng ; Xiao-Hui Wu ; Jiao Liu…
- 关键词：Molecular cloning ; Alkaline/neutral invertase ; Cassava ; Differential expression analysis ; Sucrose metabolism ; Enzyme activities
- 刊名：Plant Molecular Biology Reporter
- 出版年：2015
- 出版时间：April 2015
- 年：2015
- 卷：33
- 期：2
- 页码：304-315
- 全文大小：1,333 KB
- 参考文献：1. Bailey, TL, Williams, N, Misleh, C, Li, WW (2006) MEME: discovering and analyzing DNA and protein sequence motifs. Nucleic Acids Res 34: pp. W369-W373 CrossRef
2. Bala, N (2011) Strategies for elimination of cyanogens from cassava for reducing toxicity and improving food safety. Food Chem Toxicol 49: pp. 690-693 CrossRef
3. Bocock, P, Morse, A, Dervinis, C, Davis, J (2008) Evolution and diversity of invertase genes in Populus trichocarpa. Planta 227: pp. 565-576 CrossRef
4. Ceballos, H, Iglesias, CA, Pérez, JC, Dixon, AG (2004) Cassava breeding: opportunities and challenges. Plant Mol Biol 56: pp. 503-516 CrossRef
5. Cho, JI, Lee, SK, Ko, S, Kim, HK, Jun, SH, Lee, YH, Bhoo, SH, Lee, KW, An, G, Hahn, TR (2005) Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.). Plant Cell Rep 24: pp. 225-236 CrossRef
6. Chourey, PS, Li, QB, Cevallos-Cevallos, J (2012) Pleiotropy and its dissection through a metabolic gene Miniature1 (Mn1) that encodes a cell wall invertase in developing seeds of maize. Plant Sci 184: pp. 45-53 CrossRef
7. Dreux M, Lafosse M (1995) Chapter 13 Evaporative light scattering detection of carbohydrates in HPLC. In: Ziad El R (ed) Journal of Chromatography Library, Volume 58. Elsevier, pp 515-40
8. Flemetakis, E, Efrose, RC, Ott, T, Stedel, C, Aivalakis, G, Udvardi, MK, Katinakis, P (2006) Spatial and temporal organization of sucrose metabolism in Lotus japonicus nitrogen-fixing nodules suggests a role for the elusive alkaline/neutral invertase. Plant Mol Biol 62: pp. 53-69 CrossRef
9. Gerrits, N, Turk, SC, Dun, KP, Hulleman, SH, Visser, RG, Weisbeek, PJ, Smeekens, SC (2001) Sucrose metabolism in plastids. Plant Physiol 125: pp. 926-934 CrossRef
10. Guo, AY, Zhu, QH, Chen, X, Luo, JC (2007) GSDS: a gene structuredisplay server. Yi Chuan (Chinese) 29: pp. 1023-1026 CrossRef
11. Hayes, MA, Feechan, A, Dry, IB (2010) Involvement of abscisic acid in the coordinated regulation of a stress-inducible hexose transporter (VvHT5) and a cell wall invertase in grapevine in response to biotrophic fungal infection. Plant Physiol 153: pp. 211-221 CrossRef
12. Hyun, TK, Eom, SH, Kim, JS (2011) Genomic analysis and gene structure of the two invertase families in the domesticated apple (Malus x domestica Borkh.). Plant OMICS J Plant Mol Biol Omics 4: pp. 391-399
13. Ihemere, U, Arias–Garzon, D, Lawrence, S, Sayre, R (2006) Genetic modification of cassava for enhanced starch production. Plant Biotechnol J 4: pp. 453-465 CrossRef
14. Ji, X, Ende, W, Laere, A, Cheng, S, Bennett, J (2005) Structure, evolution, and expression of the two invertase gene families of rice. J Mol Evol 60: pp. 615-634 CrossRef
15. Lalitha, S (2000) Primer premier 5. Biotech Softw Internet Rep Comput Softw J Sci 1: pp. 270-272 CrossRef
16. Livak, KJ, Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2?ΔΔCT method. Methods 25: pp. 402-408 CrossRef
17. Martín, ML, Lechner, L, Zabaleta, EJ, Salerno, GL (2013) A mitochondrial alkaline/neutral invertase isoform (A/N-InvC) functions in developmental energy-demanding processes in Arabidopsis. Planta 237: pp. 813-822
文摘
Alkaline/neutral invertase comprises a family of enzymes that catalyze the irreversible hydrolysis of sucrose into glucose and fructose. They play a crucial role in carbohydrate partitioning and developmental processes of plants. In this study, we genome-wide identified 11 alkaline/neutral invertase family genes (MeNINVs) from the cassava genome. Based on phylogeny, MeNINVs could be classified into α and β groups. The α group genes are encoded by six exons (MeNINV1, 6, 7, 8, and 10) or four exons (MeNINV9), whereas the β group genes are encoded by four exons (MeNINV2, 3, 4, and nINV1) or five exons (MeNINV5). Their catalytic residues of motifs 2 and 4 for sucrose hydrolysis locate in all MeNINVs. qRT-PCR analysis has demonstrated that all MeNINVs are highly expressed in floral organs. During tuber development, MeNINV1, 6, 10, and nINV1 are strongly expressed in source tissues (leaves), and MeNINV1 and nINV1 also play an important role in sink organs (tuber). Activity analysis of alkaline/neutral invertase and determination of sucrose content in source and sink organs of cassava indicates that lower efficiency of sucrose hydrolysis in source organs (leaves) and higher efficiency in sink organs (tuber) are beneficial sucrose to transport from source to sink organs.