Manufacture of duck-beak bone particles with gamma-ray irradiation for bone graft

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• 作者：Se Eun Kim (1)
  Kyung Mi Shim (1)
  Seok Hwa Choi (2)
  Sung In Jeong (3)
  Jun Sik Son (4)
  Youn-Mook Lim (3)
  Seong Soo Kang (1)
  
• 关键词：xenograft ; gamma ; ray ; irradiation ; beak bone ; duck
• 刊名：Tissue Engineering and Regenerative Medicine
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：11
• 期：6
• 页码：453-457
• 全文大小：691 KB
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• 作者单位：Se Eun Kim (1)
  Kyung Mi Shim (1)
  Seok Hwa Choi (2)
  Sung In Jeong (3)
  Jun Sik Son (4)
  Youn-Mook Lim (3)
  Seong Soo Kang (1)
  
  1. College of Veterinary Medicine, Chonnam National University, Gwangju, Korea
  2. College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea
  3. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea
  4. Korea Textile Development Institute, Daegu, Korea
  
• ISSN：2212-5469

文摘

There is a growing interest in bone graft materials for enhancing bone formation. We have focused on bone xenograft using animal by-products. Among animal by-products, the duck beak was specifically used in this study for manufacture of bone graft particles. Harvested duck-beak bone was defatted, deproteinized, and then heat-treated at 350?C. Subsequently, it was ground to ?53 μm in a ceramic ball mill and irradiated with gamma rays at radiation doses of 0, 15, 20, 25, and 40 kGy. =Field emission scanning electron microscope (FE-SEM), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA) and the MTT assay were performed to investigate the effects of gamma-ray irradiation on the properties of duck-beak bone particles. Surface morphology of duck-beak bone particles by FE-SEM showed that bone particles had various sizes and similar surfaces between the 0, 15, 20, 25, and 40 kGy groups. The ATR-FTIR spectrum of all groups showed the characteristic peaks of PO4 3?/sup> and CO3 2?/sup> functional groups. Weight losses were observed and there was no significant difference in the curves depending on the radiation doses, by TGA analyses. The viabilities of human mesenchymal stem cells were 90-00% in all groups in the MTT assay. Based on our results, sterilization by gamma-ray irradiation