The Expression and Prognosis of FOXO3a and Skp2 in Human Hepatocellular Carcinoma
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- 作者：Mudan Lu (1) (2)
Jianbo Ma (4)
Wenqun Xue (2)
Chun Cheng (1)
You Wang (1) (2)
Yueming Zhao (1)
Qing Ke (1)
Haiou Liu (1)
Yonghua Liu (1)
Peng Li (4)
Xiaopeng Cui (4)
Song He (1)
Aiguo Shen (3) - 关键词：Human hepatocellular carcinoma ; FOXO protein FOXO3a ; S ; phase kinase protein (Skp2) ; Huh7 ; Immunohistochemistry (IHC) ; Prognosis
- 刊名：Pathology & Oncology Research
- 出版年：2009
- 出版时间：December 2009
- 年：2009
- 卷：15
- 期：4
- 页码：679-687
- 全文大小：381KB
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Jianbo Ma (4)
Wenqun Xue (2)
Chun Cheng (1)
You Wang (1) (2)
Yueming Zhao (1)
Qing Ke (1)
Haiou Liu (1)
Yonghua Liu (1)
Peng Li (4)
Xiaopeng Cui (4)
Song He (1)
Aiguo Shen (3)
1. Department of pathology, affiliated cancer hospital of Nantong University, medical college of Nantong University, Nantong, 226001, China
2. Wuxi Maternal and Child Health Hospital, Wuxi, 214002, China
4. Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, China
3. Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qi-xiu Road, Nantong, Jiangsu, 226001, China - ISSN：1532-2807
文摘
The forkhead box proteins (FOXO proteins) comprise a large family of functionally diverse transcription factors involved in cellular proliferation, transformation, differentiation and longevity. Recently, ubiquitination and proteasome degradation of FOXO3a have been reported. In this study, we investigated the role of FOXO3a and Skp2 in human hepatocellular carcinoma progression. Immunohistochemical analysis was performed on formalin-fixed paraffin sections of 91 specimens. Furthermore in vitro, western-blot analysis and protein stabilization studies were used to study the relationship between FOXO3a and Skp2. We found that the expression of FOXO3a was negatively related with Skp2 expression (r-??0.583; p-lt;-.05) and FOXO3a expression correlated significantly with histological grade (p--.000), cirrhosis (p--.015), and tumor size (p--.043) while Skp2 expression correlated significantly with histological grade (p--.000) and tumor size (p--.005). Kaplan-Meier analysis revealed that survival curves of low versus high expressers of FOXO3a and Skp2 showed a highly significant separation in HCC (p-lt;-.01). Our results suggested that FOXO3a and Skp2 may be considered to be important prognosis in human hepatocellular carcinoma. In vitro studies suggested that the degradation of FOXO3a may dependent on the expression of Skp2 in the proliferated Huh7 cells.