Convenient and Sensitive HPLC Method for Determination of Nitrosamines in Foodstuffs Based on Pre-column Fluorescence Labeling

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• 作者：Mei Zhao ; Guoliang Li ; Weiheng Kong ; Shuaimin Lu ; Lian Xia ; Guang Chen…
• 关键词：Nitrosamines ; HPLC ; FLD ; Fluorescence labeling ; Response surface methodology ; Foodstuffs
• 刊名：Chromatographia
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：79
• 期：7-8
• 页码：431-439
• 全文大小：1,895 KB
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• 作者单位：Mei Zhao (1)
  Guoliang Li (1) (2)
  Weiheng Kong (1)
  Shuaimin Lu (1)
  Lian Xia (1)
  Guang Chen (1)
  Xianen Zhao (1)
  Yongning Wu (2)
  Jinmao You (1) (3)
  
  1. Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, 273165, People’s Republic of China
  2. Key Laboratories of Chemical Safety and Health, China National Center for Food Safety Risk Assessment, Beijing, 100050, People’s Republic of China
  3. Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, 810001, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112

文摘

The determination of trace nitrosamines (NAs) has received more and more attention due to NAs’ strong carcinogenicity and wide existence in foodstuffs. But the convenient and sensitive method for simultaneously analyzing multiple NAs remains poorly investigated. In the present study, a new method was developed for the determination of four NAs in foodstuffs based on fluorescence labeling followed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD), which did not require complex pretreatments and expensive equipment. NAs were firstly denitrosated using hydrobromic acid-acetic acid to obtain the secondary amines. Subsequently, a fluorescence labelling procedure was immediately carried out to label the secondary amines using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC). A response surface methodology (RSM) based on a Box-Behnken design (BBD) was used to optimize the main parameters affecting the denitrosation. Under the optimal HPLC conditions, the quantitative linear range of analytes was 0.05–200 nmol L−1, and the correlation coefficients were more than 0.9998. Detection limits for all NA derivatives were achieved within the range of 1.3–2.5 ng L−1. The proposed method was successfully applied for the determination of trace NAs in several foodstuffs. This method was proven to be simple, inexpensive, sensitive, accurate and reliable for trace NA determination in foodstuffs.