Molecular analysis of a sunflower gene encoding an homologous of the B subunit of a CAAT binding factor

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• 作者：Mariangela Salvini (12) m.salvini@sns.it
  Emanuela Sani (3)
  Marco Fambrini (2)
  Laura Pistelli (4)
  Chiara Pucciariello (5)
  Claudio Pugliesi (2)
• 关键词：CCAAT binding factor – ; Gene expression – ; Methylation – ; Promoter region – ; Transcription factors – ; EMSA – ; Helianthus annuus L
• 刊名：Molecular Biology Reports
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：39
• 期：6
• 页码：6449-6465
• 全文大小：524.7 KB
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• 作者单位：1. Scuola Normale Superiore, Piazza dei Cavalieri 7, 56126 Pisa, Italy2. Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica, Universit脿 di Pisa, Via Matteotti 1B, 56124 Pisa, Italy3. Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ UK4. Dipartimento di Biologia, Unit脿 di Fisiologia Vegetale, Universit脿 di Pisa, Via Mariscoglio 34, 56124 Pisa, Italy5. PlantLab, Institute of Life Sciences, Scuola Superiore Sant鈥橝nna, Piazza Martiri della Libert脿 33, 56127 Pisa, Italy
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

A genomic DNA fragment containing the complete LEAFY COTYLEDON1-LIKE (HaL1L) gene was retrieved by chromosome walking. Its sequence was confirmed and elongated by screening a sunflower genomic DNA BAC Library. HaL1L, whose cDNA had already been sequenced and characterized, encodes a NF-YB subunit of a CCAAT box-binding factor (NF-Y) involved in the early stages of zygotic and somatic embryogenesis in the Helianthus genus. In the HaL1L 5′-flanking region, elements specific to a putative TATA-box promoter and two “CG isles” were identified. An investigation of the methylation status of these CG rich DNA regions showed that differentially methylated cytosines were recognizable in the DNA of embryos on the fifth day after pollination in comparison to leaf DNA suggesting that during plant development epigenetic regulation of HaL1L transcription was achieved by methylating cytosine residues. We also searched the HaL1L nucleotide sequence for cis-regulatory elements able to interact with other transcription factors (TFs) involved in the HaL1L regulation. Of the elements identified, one of the most intriguing is WUSATA, the target sequence for the WUSCHEL (WUS) TF, which may be part of a complex regulation network controlling embryo development. In this article, we show that the WUSATA target site, located in the intron of HaL1L, is able to bind the TF WUS. Interestingly, we found auxin and abscisic acid responsive motifs in the HaL1L promoter region suggesting that this gene may additionally by under hormonal control. Finally, the presence of a cytoplasmic polyadenylation signal downstream to the coding region indicates that this gene may also be controlled at the translation level by a temporarily making the pre-synthesized HaL1L mRNA unavailable for protein synthesis.