Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance

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• 作者：Audrey Feeney ; Christopher D Johnston ; Rodney Govender ; Jim O’Mahony…
• 关键词：Osmolytes ; Proline ; Osmotolerance ; Stress ; Cronobacter
• 刊名：Gut Pathogens
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：6
• 期：1
• 全文大小：300 KB
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• 刊物主题：Gastroenterology; Medical Microbiology; Parasitology;
• 出版者：BioMed Central
• ISSN：1757-4749

文摘

Bacteria respond to elevated osmolality by the accumulation of a range of low molecular weight molecules, known as compatible solutes (owing to their compatibility with the cells' normal physiology at high internal concentrations). The neonatal pathogen Cronobacter sakazakii is uniquely osmotolerant, surviving in powdered infant formula (PIF) which typically has a water activity (aw) of 0.2 -inhospitable to most micro-organisms. Mortality rates of up to 80% in infected infants have been recorded making C. sakazakii a serious cause for concern. In silico analysis of the C. sakazakii BAA-894 genome revealed seven copies of the osmolyte uptake system ProP. Herein, we test the physiological role of each of these homologues following heterologous expression against an osmosensitive Escherichia coli host.