Lime Pretreatment and Fermentation of Enzymatically Hydrolyzed Sugarcane Bagasse

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• 作者：Sarita C. Rabelo (1) (2)
  Rubens Maciel Filho (1)
  Aline C. Costa (1)
  
• 关键词：Lignocellulosic ; Pretreatment ; Lime ; Enzymatic hydrolysis ; Ethanol
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：169
• 期：5
• 页码：1696-1712
• 全文大小：688KB
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• 作者单位：Sarita C. Rabelo (1) (2)
  Rubens Maciel Filho (1)
  Aline C. Costa (1)
  
  1. School of Chemical Engineering, University of Campinas, UNICAMP, P.O. Box 6066, 13083-970, Campinas, S?o Paulo, Brazil
  2. Laboratório Nacional de Ciência e Tecnologia do Bioetanol -CTBE/CNPEM, Caixa Postal 6170, 13083-970, Campinas, S?o Paulo, Brazil
  
• ISSN：1559-0291

文摘

Sugarcane bagasse was subjected to lime (calcium hydroxide) pretreatment and enzymatic hydrolysis for second-generation ethanol production. A central composite factorial design was performed to determine the best combination of pretreatment time, temperature, and lime loading, as well as to evaluate the influence of enzymatic loadings on hydrolysis conversion. The influence of increasing solids loading in the pretreatment and enzymatic hydrolysis stages was also determined. The hydrolysate was fermented using Saccharomyces cerevisiae in batch and continuous mode. In the continuous fermentation, the hydrolysates were concentrated with molasses. Lime pretreatment significantly increased the enzymatic digestibility of sugarcane bagasse without the need for prior particle size reduction. In the optimal pretreatment conditions (90?h, 90?°C, 0.47?g?lime/g bagasse) and industrially realistic conditions of hydrolysis (12.7?FPU/g of cellulase and 7.3?CBU/g of β-glucosidase), 139.6?kg?lignin/ton raw bagasse and 126.0?kg hemicellulose in the pretreatment liquor per ton raw bagasse were obtained. The hydrolysate from lime pretreated sugarcane bagasse presented low amounts of inhibitors, leading to ethanol yield of 164.1?kg?ethanol/ton raw bagasse.