Detection of shigatoxigenic Escherichia coli from fecal samples of calves and cattle by molecular and serological methods

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• 作者：Saeed Sepehriseresht ; Taghi Zahraei Salehi ; Morteza Sattari ; Hasan Tadjbakhsh and Mohammad Mehdi Aslani
• 关键词：Escherichia coli ; PCR ; VTEC ; RPLA ; Shigatoxin ; Cattle
• 刊名：Comparative Clinical Pathology
• 出版年：2009
• 出版时间：February, 2009
• 年：2009
• 卷：18
• 期：1
• 页码：53-57
• 全文大小：143.1 KB

文摘

Shigatoxigenic Escherichia coli is an important cause of dysentery in domestic animals. In this study, we compared polymerase chain reaction (PCR) and serologic (through verotoxigenic escherichia coli-rapid plate latex agglutination (VTEC-RPLA) kit) methods to detect shigatoxigenic E. coli isolated from 400 fecal samples collected from cattle and calves. Primers detecting shigatoxin 1 and 2 genes (stx1 and stx2 respectively) were used. From 400 fecal samples, 328 (82 % ) and 72 (18 % ) were collected from diarrheic and non-diarrheic calves respectively. Thirty-four isolates (8.5 % ) were positive in their PCR results performed on their total DNA of which 24 (70.5 % ) carried stx1 gene, eight (23.5 % ) carried stx2 gene and two (6 % ) carried both stx1 and stx2 genes. From 34 shigatoxigenic isolates, 27 (79.5 % ) were collected from diarrheic and seven (20.5 % ) were collected from non-diarrheic calves. All of 400 isolates were evaluated through verotoxigenic escherichia coli-rapid plate latex agglutination for shigatoxin production. There was 100 % concordance between PCR and VTEC-RPLA results.