Virulence profiles of enterotoxigenic, shiga toxin and enteroaggregative Escherichia coli in South African pigs

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• 作者：Ramadimetja Prescilla Mohlatlole (1)
  Evelyn Madoroba (2)
  Farai Catherine Muchadeyi (3)
  Michael Chimonyo (4)
  Arnold Tapera Kanengoni (5)
  Edgar Farai Dzomba (1)
  
• 关键词：Diarrhoea ; Oedema ; Escherichia coli ; Pigs
• 刊名：Tropical Animal Health and Production
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：45
• 期：6
• 页码：1399-1405
• 全文大小：193KB
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• 作者单位：Ramadimetja Prescilla Mohlatlole (1)
  Evelyn Madoroba (2)
  Farai Catherine Muchadeyi (3)
  Michael Chimonyo (4)
  Arnold Tapera Kanengoni (5)
  Edgar Farai Dzomba (1)
  
  1. Discipline of Genetics, School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville, 3209, Pietermaritzburg, South Africa
  2. Bacteriology Section, Agricultural Research Council–Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort, 0110, South Africa
  3. Biotechnology Platform, Agricultural Research Council, Private Bag X5, Onderstepoort, 0110, South Africa
  4. Discipline of Animal and Poultry Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville, 3209, Pietermaritzburg, South Africa
  5. Agricultural Research Council, Animal Production Institute, Private Bag X2, 0062, Irene, South Africa
  

文摘

Enterotoxigenic Escherichia coli (ETEC) and shiga toxin E. coli (STEC) are important causes of colibacillosis in piglets. Recently, enteroaggregative E. coli heat-stable enterotoxin 1 (EAST-1) has been implicated in pig diarrhoea. This study investigated the prevalence of enterotoxin [heat-labile toxins (LT), heat-stable toxin a (STa), heat-stable toxin b (STb)], shiga toxins (Stx1, Stx2, Stx2e), enteroaggregative heat-stable E. coli (EAST-1), associated fimbriae (F4, F5, F6, F41, F18ab, F18ac) and non-fimbrial adhesins [adhesin involved in diffuse adherence 1 (AIDA-1), attaching and effacing factor, porcine attaching- and effacing-associated factor] in South African pigs. A total of 263 E. coli strains were isolated from Landrace (n--4), Large White (n--26), Duroc (n--8) and indigenous (n--5) breeds of piglets aged between 9 and 136?days. PCR was used in the analysis. Virulent genes were detected in 40.3?% of the isolates, of which 18.6, 0.4 and 17.5?% were classified as ETEC, STEC and enteroaggregative E. coli (EAEC), respectively. Individual genes were found in the following proportions: STb (19.01?%), LT (0.4?%), STa (3.4?%), St2xe (1.1?%) and EAST-1 (20.2?%) toxins. None of the tested fimbriae were detected in ETEC and STEC isolates. About one third of the ETEC and STEC isolates was tested negative for both fimbrial and non-fimbrial adhesins. Twenty-five pathotypes from ETEC-, EAEC- and STEC-positive strains were identified. Pathotypes EAST-1 (30.2?%), STb (13.2?%) and STb/AIDA-1 (10.4?%) were most prevalent. The study provided insight on possible causes of colibacillosis in South African pigs.