TIP30 directly binds p53 tumor suppressor protein in vitro
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- 作者：Si-Hyung Lee (1) (2)
Sung-Kyu Ju (3)
Tae-Young Lee (4)
Sung-Ho Huh (2)
Kyou-Hoon Han (1) (5) - 关键词：GST pull ; down assay ; p53 ; protein ; protein interaction ; surface plasmon resonance ; TIP30
- 刊名：Molecules and Cells
- 出版年：2012
- 出版时间：November 2012
- 年：2012
- 卷：34
- 期：5
- 页码：495-500
- 全文大小：464KB
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Sung-Kyu Ju (3)
Tae-Young Lee (4)
Sung-Ho Huh (2)
Kyou-Hoon Han (1) (5)
1. Biomedical Translational Research Center, Division of Convergent Biomedical Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea
2. Department of Biochemistry, Graduate School, Chungnam National University, Daejeon, 305-764, Korea
3. Department of Bioscience and Biotechnology, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon, 305-764, Korea
4. Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea
5. Department of Bioinformatics, University of Science and Technology, Daejeon, 305-333, Korea - ISSN：0219-1032
文摘
TIP30 (30 kDa HIV-1 TAT-interacting protein), also called HTATIP2 or CC3, is a tumor suppressor protein that acts as an angiogenesis inhibitor. TIP30 blocks nuclear import of the mRNA-binding protein HuR, and thereby promotes the cytoplasmic accumulation of HuR by binding to importin-β, which is known to facilitate the cytoplasm-tonuclear transport of HuR. Accumulation of HuR in the cytoplasm, in turn, enhances the expression of the transcription factor p53, a tumor suppressor that plays an essential role in preserving genome stability and inhibiting cancer growth. In addition to such a post-transcriptional mechanism via which TIP30 increases the p53 level, it has been proposed that TIP30 may regulate p53 protein at the protein level by directly binding to it. In order to investigate the possibility of direct interaction between p53 and TIP30, we have used on three functional regions in p53 and examined their interactions with TIP30 using GST pull-down assay and surface plasmon resonance technique. The results show that that TIP30 binds to the DNA-binding domain and the C-terminal domain of p53.