Ganoderma lucidum polysaccharide extract inhibits hepatocellular carcinoma growth by downregulating regulatory T cells accumulation and function by inducing microRNA-125b

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• 作者：Aimei Li (1) (2)
  Xuanyu Shuai (1)
  Zhijun Jia (2)
  Hangyu Li (3)
  Xiubin Liang (5)
  Dongming Su (1) (4) (5)
  Wanhua Guo (2)
  
  1. Department of Pathology ; Nanjing Medical University ; Nanjing ; China
  2. Department of Nuclear Medicine ; The Affiliated Drum Tower Hospital of NanJing University ; Zhongshan Road ; Nanjing ; 210008 ; China
  3. Department of General Surgery ; Shengjing Hospital Affiliated to China Medical University ; Shenyang ; China
  5. Center of Metabolic Disease Research ; Nanjing Medical University ; 140 Hanzhong Road ; Nanjing ; 210029 ; China
  4. Center of Cellular Therapy ; The Second Affiliated Hospital of Nanjing Medical University ; Nanjing ; China
  
• 关键词：Ganoderma lucidum polysaccharides ; Hepatocellular carcinoma ; Regulatory T cell ; Effector T cell ; miR ; 125b
• 刊名：Journal of Translational Medicine
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：13
• 期：1
• 全文大小：1,797 KB
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• 刊物主题：Biomedicine general; Medicine/Public Health, general;
• 出版者：BioMed Central
• ISSN：1479-5876

文摘

Background Ganoderma lucidum polysaccharides (GLPS) have been used as traditional Chinese medicine for their properties of cancer prevention and immunomodulation. However, it is unclear whether GLPS has therapeutic effect on anti-hepatocellular carcinoma (HCC) in vivo. In this study, the effect of GLPS and their impact on the balance of regulatory T cell (Treg) and effector T cell (Teff) was measured in a model of hepatoma-bearing mice. Methods The effect of GLPS and their impact on the balance of regulatory T cell (Treg) and effector T cell (Teff) were measured in a model of hepatoma-bearing mice. Real-time PCR detected the levels of MicroRNAs (miRNAs) and mRNA. The effects of Tregs on Teff proliferation were determined via suppression assay. The mircroRNA-125b (miR-125b) inhibitor was used to down-regulate miR-125b expression. Results GLPS significantly suppressed tumor growth in hepatoma-bearing mice associated with an increase of the ratio of Teffs to Tregs. Moreover, GLPS eliminate Treg suppression of Teff proliferation with an increase in IL-2 secretion. Addition of GLPS to treat T cells inhibited Notch1 and FoxP3 expression through increase of miR-125b expression. In hepatoma-bearing mice, miR-125b inhibitor obviously abolished the effect of GLPS on tumor growth. Conclusions This finding provides the novel evidence for GLPS on inhibition of HCC through miR-125b inhibiting Tregs accumulation and function.