SYK is a target of lymphocyte-derived microparticles in the induction of apoptosis of human retinoblastoma cells

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• 作者：Qian Qiu ; Chun Yang ; Wei Xiong ; Houda Tahiri ; Mathieu Payeur…
• 关键词：Retinoblastoma ; Lymphocyte ; derived microparticles ; Spleen tyrosine kinase ; Apoptosis ; p53–p21 pathway
• 刊名：Apoptosis
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：20
• 期：12
• 页码：1613-1622
• 全文大小：2,555 KB
• 参考文献：1.Friend SH, Bernards R, Rogelj S, Weinberg RA, Rapaport JM, Albert DM, Dryja TP (1986) A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature 323:643-46CrossRef PubMed
  2.Zhang J, Benavente CA, McEvoy J, Flores-Otero J, Ding L, Chen X, Ulyanov A, Wu G, Wilson M, Wang J, Brennan R, Rusch M, Manning AL, Ma J, Easton J, Shurtleff S, Mullighan C, Pounds S, Mukatira S, Gupta P, Neale G, Zhao D, Lu C, Fulton RS, Fulton LL, Hong X, Dooling DJ, Ochoa K, Naeve C, Dyson NJ, Mardis ER, Bahrami A, Ellison D, Wilson RK, Downing JR, Dyer MA (2012) A novel retinoblastoma therapy from genomic and epigenetic analyses. Nature 481:329-34PubMed Central PubMed
  3.Laurie NA, Donovan SL, Shih CS, Zhang J, Mills N, Fuller C, Teunisse A, Lam S, Ramos Y, Mohan A, Johnson D, Wilson M, Rodriguez-Galindo C, Quarto M, Francoz S, Mendrysa SM, Guy RK, Marine JC, Jochemsen AG, Dyer MA (2006) Inactivation of the p53 pathway in retinoblastoma. Nature 444:61-6CrossRef PubMed
  4.Symonds H, Krall L, Remington L, Saenz-Robles M, Lowe S, Jacks T, Van Dyke T (1994) p53-dependent apoptosis suppresses tumor growth and progression in vivo. Cell 78:703-11CrossRef PubMed
  5.El-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B (1993) WAF1, a potential mediator of p53 tumor suppression. Cell 75:817-25CrossRef PubMed
  6.Chang LJ, Eastman A (2012) Decreased translation of p21waf1 mRNA causes attenuated p53 signaling in some p53 wild-type tumors. Cell cycle 11:1818-826PubMed Central CrossRef PubMed
  7.Yang C, Mwaikambo BR, Zhu T, Gagnon C, Lafleur J, Seshadri S, Lachapelle P, Lavoie JC, Chemtob S, Hardy P (2008) Lymphocytic microparticles inhibit angiogenesis by stimulating oxidative stress and negatively regulating VEGF-induced pathways. Am J Physiol Regul Integr Comp Physiol 294:R467–R476CrossRef PubMed
  8.Yang C, Xiong W, Qiu Q, Shao Z, Hamel D, Tahiri H, Leclair G, Lachapelle P, Chemtob S, Hardy P (2012) Role of receptor-mediated endocytosis in the antiangiogenic effects of human T lymphoblastic cell-derived microparticles. Am J Physiol Regul Integr Comp Physiol 302:R941–R949CrossRef PubMed
  9.Yang C, Gagnon C, Hou X, Hardy P (2010) Low density lipoprotein receptor mediates anti-VEGF effect of lymphocyte T-derived microparticles in Lewis lung carcinoma cells. Cancer Biol Ther 10:448-56PubMed Central CrossRef PubMed
  10.Yang C, Xiong W, Qiu Q, Tahiri H, Superstein R, Carret AS, Sapieha P, Hardy P (2014) Anti-proliferative and anti-tumour effects of lymphocyte-derived microparticles are neither species- nor tumour-type specific. J Extracell Vesicles. doi:10.-402/?jev.?v3.-3034 PubMed Central PubMed
  11.Mwaikambo BR, Yang C, Chemtob S, Hardy P (2009) Hypoxia up-regulates CD36 expression and function via hypoxia-inducible factor-1- and phosphatidylinositol 3-kinase-dependent mechanisms. J Biol Chem 284:26695-6707PubMed Central CrossRef PubMed
  12.Qiu Q, Xiong W, Yang C, Gagnon C, Hardy P (2013) Lymphocyte-derived microparticles induce bronchial epithelial cells-pro-inflammatory cytokine production and apoptosis. Mol Immunol 55:220-30CrossRef PubMed
  13.Huber J, Vales A, Mitulovic G, Blumer M, Schmid R, Witztum JL, Binder BR, Leitinger N (2002) Oxidized membrane vesicles and blebs from apoptotic cells contain biologically active oxidized phospholipids that induce monocyte-endothelial interactions. Arterioscler Thromb Vasc Biol 22:101-07CrossRef PubMed
  14.Qiu Q, Xiong W, Yang C, Dai X, Dan X, Yang Z, Jiao Y, Xiang Y, Liu G, Hardy P (2014) Lymphocyte-derived microparticles induce apoptosis of airway epithelial cells through activation of p38 MAPK and production of arachidonic acid. Apoptosis 19:1113-127CrossRef PubMed
  15.Mocsai A, Ruland J, Tybulewicz VL (2010) The SYK tyrosine kinase: a crucial player in diverse biological functions. Nat Rev Immunol 10:387-02CrossRef PubMed
  16.Bailet O, Fenouille N, Abbe P, Robert G, Rocchi S, Gonthier N, Denoyelle C, Ticchioni M, Ortonne JP, Ballotti R, Deckert M, Tartare-Deckert S (2009) Spleen tyrosine kinase functions as a tumor suppressor in melanoma cells by inducing senescence-like growth arrest. Cancer Res 69:2748-756PubMed Central CrossRef PubMed
  17.Kaplan D, Meyerson HJ, Li X, Drasny C, Liu F, Costaldi M, Barr P, Lazarus HM (2010) Correlation between ZAP-70, phospho-ZAP-70, and phospho-Syk expression in leukemic cells from patients with CLL. Cytometry B 78:115-22
  18.Bieging KT, Mello SS, Attardi LD (2014) Unravelling mechanisms of p53-mediated tumour suppression. Nat Rev 14:359-70CrossRef
  19.Hong H, Takahashi K, Ichisaka T, Aoi T, Kanagawa O, Nakagawa M, Okita K, Yamanaka S (2009) Suppression of induced pluripotent stem cell generation by the p53–p21 pathway. Nature 460:1132-135PubMed Central CrossRef PubMed
  20.Roninson IB (2002) Oncogenic functions of tumour suppressor p21(Waf1/Cip1/Sdi1): association with cell senescence an
• 作者单位：Qian Qiu (2)
  Chun Yang (1)
  Wei Xiong (2)
  Houda Tahiri (1)
  Mathieu Payeur (3)
  Rosanne Superstein (4)
  Anne-Sophie Carret (1)
  Patrick Hamel (4)
  Benjamin Ellezam (5)
  Bussières Martin (1)
  Mark Vezina (6)
  Przemyslaw Sapieha (4)
  Guoxiang Liu (2)
  Pierre Hardy (1) (7)
  
  2. Department of Pulmonology, Southwest Hospital, Third Military Medical University, Chongqing, China
  1. Departments of Pediatrics, Hematology-Oncology and Pharmacology, University of Montréal, Montreal, QC, Canada
  3. Medical School, University of Montréal, Montreal, QC, Canada
  4. Department of Ophthalmology, University of Montréal, Montreal, QC, Canada
  5. Department of Pathology and Cell Biology, University of Montréal, Montreal, QC, Canada
  6. Charles River Laboratories Preclinicals Services, Senneville, QC, Canada
  7. Research Center of CHU Sainte-Justine, 3175 C?te-Sainte-Catherine, Room 2714, Montreal, QC, H3T 1C5, Canada
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Cancer Research
  Cell Biology
  Biochemistry
  Virology
  
• 出版者：Springer Netherlands
• ISSN：1573-675X

文摘

Retinoblastoma (Rb) is an aggressive childhood cancer of the developing retina. This disease is associated with epigenetic deregulation of several cancer pathways including upregulation of the proto-oncogene spleen tyrosine kinase (SYK). We have previously demonstrated that lymphocyte-derived microparticles (LMPs) possess strong cytotoxic effect on cancer cells. This report demonstrated that LMPs have potent pro-apoptotic properties on human Rb cells, which was associated with a strong reduction of SYK expression. Perturbing SYK activity in Rb cells induced cell apoptosis and upregulated expression of p53 and p21. Interestingly, inhibition of p53 or knockdown of p21, abolished LMP-induced caspase-3 activity and cell death. Blocking oxidized phospholipid-rich LMPs with a specific antibody significantly prevented LMP-induced Rb apoptosis and reversed the expression patterns of SYK, p53, p21. In summary, our results suggest that LMPs are important pro-apoptotic regulators for Rb cells through reduction of SYK expression and upregulation of the p53–p21 pathway which ultimately activates caspase-3. These data may open unexpected avenues for the development of LMPs as a novel therapeutic strategy that would be particularly useful and relevant for the treatment of Rb. Keywords Retinoblastoma Lymphocyte-derived microparticles Spleen tyrosine kinase Apoptosis p53–p21 pathway