Characterization and expression analysis of B Cell receptor accessory molecule CD79 gene in humphead snapper (Lutjanus sanguineus)

详细信息    查看全文

• 作者：Yucong Huang ; Xiuying Yan ; Shuanghu Cai ; Jia Cai…
• 关键词：Lutjanus sanguineus ; immune response ; BCR ; CD79α ; CD79β
• 刊名：Journal of Ocean University of China
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：15
• 期：2
• 页码：318-326
• 全文大小：811 KB
• 参考文献：Braun, D., Caramalho, I., and Demengeot, J., 2002. IFN-a/ß enhances BCR-dependent B cell responses. International Immunology, 14(4): 411–419.CrossRef
  Burkhardt, A. L., Costa, T., Misulovin, Z., Stealy, B., Bolen, J. B., and Nußsenzweig, M. C., 1994. Iga and Igß are functionally homologous to the signaling proteins of the T-cell receptor. Molecular and Cellular Biology, 14(2): 1095–1103.CrossRef
  Cai, S. H., Huang, Y. C., Lu, Y. S., Wu, Z. H., Wang, B., Tang, J. F., and Jian, J. C., 2013. Expression and immunogenicity analysis of accessory colonization factor A from Vibrio alginolyticus strain HY9901. Fish and Shellfish Immunology, 34(2): 454–462.CrossRef
  Chu, P. G., and Arber, D. A., 2001. CD79: A review. Applied Immunohistochemistry and Molecular Morphology, 9(2): 97–106.
  Darawiroj, D., Kondo, H., Hirono, I., and Aoki, T., 2008. Immune-related gene expression profiling of yellowtail (Seriola quinqueradiata) kidney cells stimulated with ConA and LPS using microarray analysis. Fish and Shellfish Immunology, 24(2): 260–266.CrossRef
  DeFranco, A. L., 1993. Structure and function of the B cell antigen receptor. Annual Review of Cell and Developmental Biology, 9: 377–410.CrossRef
  Duncan, L., Webster, K., Gupta, V., Nair, S., and Deane, E., 2010. Molecular characterisation of the CD79a and CD79b subunits of the B cell receptor complex in the gray shorttailed opossum (Monodelphis domestica) and tammar wallaby (Macropus eugenii): Delayed B cell immunocompetence in marsupial neonates. Veterinary Immunology and Immunopathology, 136 (3-4): 235–247.CrossRef
  Dylke, J., Lopes, J., Dang-Lawson, M., Machtaler, S., and Matsuuchi, L., 2007. Role of the extracellular and transmembrane domain of Ig-a/ß in assembly of the B cell antigen receptor (BCR). Immunology Letters, 112(1): 47–57.CrossRef
  Ferrari, S., Lougaris, V., Caraffi, S., Zuntini, R., Yang, J., Soresina, A., Meini, A., Cazzola, G., Roßsi, C., Reth, M., and Plebani, A., 2007. Mutations of the Igß gene cause agammaglobulinemia in man. The Journal of Exprimental Medicine, 204(9): 2047–2051.CrossRef
  Grøntvedt, R. N., and Espelid, S., 2003. Immunoglobulin producing cells in the spotted wolffish (Anarhichas minor Olafsen): Localization in adults and during juvenile development. Developmental and Comparative Immunology, 27 (6-7): 569–578.CrossRef
  Guselnikov, S. V., Bell, A., Najakshin, A. M., Robert, J., and Taranin, A. V., 2003. Signaling FcR and TCR subunit homologs in the amphibian Xenopus laevis. Developmental and Comparative Immunology, 27(8): 727–733.CrossRef
  Ha, H. J., Kubagawa, H., and Burrows, P. D., 1992. Molecular cloning and expression pattern of a human gene homologous to the murine mb-1 gene. The Journal of Immunology, 148(5): 526–1531.
  Halaby, D. M., and Mornon, J. P., 1998. The immunoglobulin superfamily: An insight on its tissular, species, and functional diversity. Journal of Molecular Evolution, 46(4): 389–400.CrossRef
  Hermanson, G. G., Eisenberg, D., Kincade, P. W., and Wall, R., 1988. B29: A member of the immunoglobulin gene superfamily exclusively expressed on B-lineage cells. Proceedings of the National Academy of Sciences, 85(18): 6890–6894.CrossRef
  Herren, B., and Burrows, P. D., 2002. B cell-restricted human mb-1 gene: Expression, function, and lineage infidelity. Immunological Research, 26 (1-3): 35–43.CrossRef
  Hombach, J., Tsubata, T., Leclercq, L., Stappert, H., and Reth, M., 1990. Molecular-components of the B-cell antigen receptor complex of the IgM class. Nature, 343(6260): 760–762.CrossRef
  Kabak, S., Skaggs, B. J., Gold, M. R., Affolter, M., West, K. L., Foster, M. S., Siemasko, K., Chan, A. C., Aebersold, R., and Clark, M. R., 2002. The direct recruitment of BLNK to immunoglobulin a couples the B-cell antigen receptor to distal signaling pathways. Molecular and Cellular Biology, 22(8): 2524–235.CrossRef
  Kaloff, C. R., and Haas, I. G., 1995. Coordination of immunoglobulin chain folding and immunoglobulin chain assembly is essential for the formation of functional IgG. Immunity, 2(6): 629–637.CrossRef
  Katsukura, H., Murakami, R., Chijiiwa, Y., Otsuka, A., Tanaka, M., Nakashima, K., and Ono, M., 2001. Structure of the ß-chain (B29) gene of the chicken B-cell receptor and conserved collinearity with genes for potential skeletal muscle sodium channel and growth hormone. Immunogenetics, 53(9): 770–775.CrossRef
  Kehrer, S. R., Hannan, C. M., and Raison, R. L., 1998. Identification of a subpopulation of leucocytes from the rainbow trout (Oncorhynchus mykiss) responsive to pokeweed mitogen. Fish and Shellfish Immunology, 8(6): 477–487.CrossRef
  Keightley, R. G., Cooper, M. D., and Lawton, A. R., 1976. The T cell dependence of B cell differentiation induced by pokeweed mitogen. The Journal of Immunology, 117: 1538–1544.
  Kraus, M., Pao, L. I., Reichlin, A., Hu, Y., Canono, B., Cambier, J. C., Nußsenzweig, M. C., and Rajewsky, K., 2001. Interference with immunoglobulin (Ig)a immunoreceptor tyrosinebased activation motif (ITAM) phosphorylation modulates or blocks B cell development, depending on the availability of an Igß cytoplasmic tail. The Journal of Experimental Medicine, 194(4): 455–469.CrossRef
  Lee, S. J., Kim, S. J., Park, C. G., Park, J., Kim, J. H., and Chun, T., 2008. Molecular cloning and expression analysis of pig CD79a. Veterinary Immunology and Immunopathology, 125 (3-4): 368–374.CrossRef
  Li, R., Wang, T., Bird, S., Zou, J., Dooley, H., and Secombes, C. J., 2013. B cell receptor accessory molecule CD79a: Characterisation and expression analysis in a cartilaginous fish, the spiny dogfish (Squalus acanthias). Fish and Shellfish Immunology, 34(6): 1404–1415.CrossRef
  Livak, K. J., and Schmittgen, T. D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-??CT Method. Methods, 25(4): 402–408.CrossRef
  Luger, D., Yang, Y. A., Raviv, A., Weinberg, D., Banerjee, S., Lee, M. J., Trepel, J., Yang, L., and Wakefield, L. M., 2013. Expression of the B-cell receptor component CD79a on immature myeloid cells contributes to their tumor promoting effects. PLoS One, 8 (10): e76115.CrossRef
  Minegishi, Y., Coustan-Smith, E., Rapalus, L., Ersoy, F., Campana, D., and Conley, M. E., 1999. Mutations in Iga (CD79a) result in a complete block in B-cell development. The Journal of Clinical Investigation, 104(8): 1115–1121.CrossRef
  Patel, S., Sørhus, E., Fiksdal, I. U., Espedal, P. G., Bergh, O., Rødseth, O. M., Morton, H. C., and Nerland, A. H., 2009. Ontogeny of lymphoid organs and development of IgMbearing cells in Atlantic halibut (Hippoglossus hippoglossus L.). Fish and Shellfish Immunology, 26(3): 385–395.CrossRef
  Patterson, H. C., Kraus, M., Kim, Y. M., Ploegh, H., and Rajewsky, K., 2006. The B cell receptor promotes B cell activation and proliferation through a non-ITAM tyrosine in the Iga cytoplasmic domain. Immunity, 25(1): 55–65.CrossRef
  Pike, K. A., Iacampo, S., Friedmann, J. E., and Ratcliffe, M. J., 2004. The cytoplasmic domain of Iga is necessary and sufficient to support efficient early B cell development. Journal of Immunol, 72(4): 2210–2218.CrossRef
  Reth, M., 1989. Antigen receptor tail clue. Nature, 338(6214): 383–384.CrossRef
  Reth, M., 1992. Antigen receptors on B lymphocytes. Annual Review of Immunology, 10: 97–121.CrossRef
  Reth, M., 1995. The B-cell antigen receptor complex and coreceptors. Immunology Today, 16(7): 310–313.CrossRef
  Sahoo, M., Edholm, E. S., Stafford, J. L., Bengtén, E., Miller, N. W., and Wilson, M., 2008. B cell receptor accessory molecules in the channel catfish, Ictalurus punctatus. Development and Comparative Immunology, 32(11): 1385–1397.CrossRef
  Sakaguchi, N., Kashiwamura, S., Kimoto, M., Thalmann, P., and Melchers, F., 1988. B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-1ike properties. The EMBO Journal, 7(11): 3457–3464.
  Sakai, M., Kono, T., and Savan, R., 2005. Identification of expressed genes in carp (Cyprinus carpio) head kidney cells after in vitro treatment with immunostimulants. Journal of Developmental Biology (Basel), 121: 45–51.
  Sanchez, M., Misulovin, Z., Burkhardt, A. L., Mahajan, S., Costa, T., Franke, R., Bolen, J. B., and Nußsenzweig, M., 1993. Signal transduction by immunoglobulin is mediated through Iga and Igß. The Journal of Experimental Medicine, 178(3): 1049–1055.CrossRef
  Schrder, M. B., Flao, E., and Pilstrm, L., 1998. Localisation of Ig heavy chain mRNA positive cells in Atlantic cod (Gadus morhuabL.) tissues identified by in situ hybridization. Fish and Shellfish Immunology, 8(1): 45–50.
  Siegers, G. M., Yang, J., Duerr, C. U., Nielsen, P. J., Reth, M., and Schamel, W. W., 2006. Identification of disulfide bonds in the Ig-a/Ig-ß component of the B cell antigen receptor using the Drosophila S2 cell reconstitution system. International Immunology, 18(9): 1385–1396.CrossRef
  Swanson, C. L., Wilson, T. J., Strauch, P., Colonna, M., Pelanda, R., and Torres, R. M., 2010. Type I IFN enhances follicular B cell contribution to the T cell-independent antibody response. The Journal of Experimental Medicine, 207(7): 1485–5000.CrossRef
  Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30(12): 2725–2729.CrossRef
  Taniguchi, T., and Takaoka, A., 2002. The interferon-a/ß system in antiviral responses: A multimodal machinery of gene regulation by the IRF family of transcription factors. Current Opinion in Immunology, 14(1): 111–116.CrossRef
  Thompson, A. A., Do, H. N., Saxon, A., and Wall, R., 1999. Widespread B29 (CD79b) gene defects and loss of expression in chronic lymphocytic leukemia. Leukemia & Lymphoma, 32 (5-6): 561–569
  Van Noesel, C. J., Van Lier, R. A., Cordell, J. L., Tse, A. G., Van Schijndel, G. M., de Vries, E. F., Mason, D. Y., and Borst, J., 1991. The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. The Jouranl of Immunology, 146(11): 3881–3888.
  Wang, H., Diamond, R. A., and Rothenberg, E. V., 1998. Croßslineage expression of Igß(B29) in thymocytes: Positive and negative gene regulation to establish T cell identity. Proceedings of the National Academy of Sciences, 95(12): 6831–6836.CrossRef
  Wang, P. C., Huang, J. P., Tsai, M. A., Cheng, S. Y., Tsai, S. S., Chen, S. D., Chen, S. P., Chiu, S. H., Liaw, L. L., Chang, L. T., and Chen, S. C., 2005). Systemic infection of Kudoa lutjanus n. sp. (Myxozoa: Myxosporea) in red snapper Lutjanus erythropterus from Taiwan. Disease of Aquatic Organisms, 67 (1-2): 115–124.CrossRef
  Williams, A. F., and Barclay, A. N., 1988. The immunoglobulin superfamily-domains for cell surface recognition. Annual Review of Immunology, 6: 381–405.CrossRef
  Youn, H. Y., Goitsuka, R., Kato, H., Mason, D. Y., Watari, T., Tsujimoto, H., and Hasegawa, A., 1996. Molecular cloning of bovine mb-1 cDNA. Veterinary Immunology and Immunopathology, 52(3): 191–200.CrossRef
  Zhang, X. Z., Wu, Z. H., Yang, S. P., Pang, H. Y., Jian, J. C., and Lu, Y. S., 2011. Expression pattern of heat-shock cognate 70 gene of humphead snapper, Lutjanus sanguineus (Cuvier), infected by Vibrio harveyi. Journal of Fish Disease, 34(10): 719–729.CrossRef
  
• 作者单位：Yucong Huang (1) (2) (3) (4)
  Xiuying Yan (3) (4)
  Shuanghu Cai (3) (4)
  Jia Cai (3) (4)
  Jichang Jian (3) (4)
  Yishan Lu (3) (4)
  Jufen Tang (3) (4)
  Zaohe Wu (4) (5)
  
  1. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, P. R. China
  2. University of Chinese Academy of Sciences, Beijing, 100049, P. R. China
  3. Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, P. R. China
  4. Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals of Guangdong, Zhanjiang, 524088, P. R. China
  5. Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, P. R. China
  
• 刊物主题：Oceanography; Meteorology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-5021

文摘

CD79, a key component of the B cell antigen receptor complex, is composed of CD79α(Igα) and CD79β(Igβ) encoded by mb-1 and B29 respectively, and plays an important role in B cell signaling. In this study, we isolated and characterized mb-1 and B29 from humphead snapper (Lutjanus sanguineus). Their tissue distribution and expression profiles after stimulations in vitro and in vivo were also investigated. The humphead snapper mb-1 and B29 contain open reading frames of 684 bp and 606 bp, encoding 227 amino acids and 201 amino acids, respectively. Both CD79α and CD79β possess signal peptide, extracellular Ig domain, transmembrane region and immunoreceptor tyrosine kinase activation motif (ITAM). Mb-1 is highly expressed in lymphoid organs (thymus, posterior kidney and spleen) and mucosal-associated lymphoid tissues (gill and intestine), while B29 is mainly detected in posterior kidney, spleen, gill and skin. Furthermore, transcription of mb-1 and B29 in head kidney leucocytes was up-regulated following lipopolysaccharide (LPS), pokeweed mitogen (PWM), and polyinosinic-polycytidylic acid (PolyI:C) stimulation, respectively, and their expression level in anterior kidney and spleen was also increased after challenged with formalin-inactived Vibrio harveyi. These results indicated that humphead snapper CD79 molecule might play an important role in immune response to pathogen infection. Keywords Lutjanus sanguineus immune response BCR CD79α CD79β