Immune response induced by candidate Sarcoptes scabiei var. cuniculi DNA vaccine encoding paramyosin in mice

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• 作者：Xiaobin Gu (1) (4)
  Yue Xie (1)
  Shuxian Wang (1)
  Xuerong Peng (2)
  Songjia Lai (3)
  Guangyou Yang (1)
  
• 关键词：Sarcoptes scabiei ; Paramyosin ; DNA vaccine ; Immune response ; Mice
• 刊名：Experimental and Applied Acarology
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：63
• 期：3
• 页码：401-412
• 全文大小：
• 参考文献：1. Abou-Elhakam H, Rabee I, Deeb SE, Amir AE (2013) Protection against / Fasciola gigantica using paramyosin antigen as a candidate for vaccine production. Pak J Biol Sci 16:1449-458. doi:10.3923/pjbs.2013.1449.1458 CrossRef
  2. Aiello SE, Mays A, Amstutuz HZ (1998) Rabbits. In: Aiello SE (ed) Merck veterinary manual. Merck Inc., New Jersey, pp 1386-396
  3. Alasaad S, Soglia D, Maione S, Sartore S, Soriguer RC, Pérez JM, Rasero R, Rossi L (2009) Effectiveness of the postponed isolation (post-frozen isolation) method for PCR-quality / Sarcoptes mite gDNA. Exp Appl Acarol 47:173-78. doi:10.1007/s.10493-008-9196.-0 CrossRef
  4. Arlian LG (1989) Biology, host relations, and epidemiology of / Sarcoptes scabiei. Annu Rev Entomol 34:139-59. doi:10.1146/annurev.en.34.010189.001035 CrossRef
  5. Arlian LG, Morgan MS, Vyszenskimoher DL, Stemmer BL (1994a) / Sarcoptes scabiei: the circulating antibody response and induced immunity to scabies. Exp Parasitol 78:37-0. doi:10.1006/expr.1994.1004 CrossRef
  6. Arlian LG, Rapp CM, Vyszenskimoher DL, Morgan MS (1994b) / Sarcoptes scabiei: histopathological changes associated with acquisition and expression of host immunity to scabies. Exp Parasitol 78:51-3. doi:10.1006/expr.1994.1005 CrossRef
  7. Arlian LG, Rapp CM, Morgan MS (1995) Resistance and immune response in scabies-infested hosts immunized with / Dermatophagoides mites. Am J Trop Med Hyg 52(6):539-45
  8. Arlian LG, Morgan MS, Rapp CM, Vyszenski-Moher DL (1996) The development of protective immunity in canine scabies. Vet Parasitol 62:133-42. doi:10.1016/0304-4017(95)00854-3 CrossRef
  9. Arlian LG, Fall N, Morgan MS (2007) In / vivo evidence that / Sarcoptes scabiei (Acari: sarcoptidae) is the source of molecules that modulate splenic gene expression. J Med Entomol 44:1054-063. doi:10.1603/0022-2585(2007)44[1054:IVETSS]2.0.CO;2 CrossRef
  10. Currie BJ, Harumal P, McKinnon M, Walton SF (2004) First documentation of in vivo and in vitro ivermectin resistance in / Sarcoptes scabiei. Clin Infect Dis 39:8-2. doi:10.1086/421776 CrossRef
  11. Donnelly JJ, Ulmer JB, Liu MA (1996) DNA vaccin Life Sci. 15:163-72. doi:10.1016/S0024-3205(96)00502-4 CrossRef
  12. Ferreira CAS, Barbosa MC, Silveira TCL, Valenzuela JG, Dasilvavazjr I, Masuda A (2002) cDNA cloning, expression and characterization of a / Boophilus microplus paramyosin. Parasitology 125:265-74. doi:10.1017/S0031182002002019 CrossRef
  13. Flanigan TP, King CH, Lett RR, Nanduri J, Mahmoud AA (1989) Induction of resistance to / Schistosoma mansoni infection in mice by purified parasite paramyosin. J Clin Invest. 83:1010-014. doi:10.1172/JCI113942 CrossRef
  14. Gu XB, Yang GY (2008) A study on the genetic relationship of mites in the genus / Sarcoptes (Acari: Sarcoptidae) in China. Int J Acarol. 34:183-90. doi:10.1080/01647950808683722 CrossRef
  15. Halley BA, VandenHeuvel WJA, Wislocki PG (1993) Environmental effects of the usage of avermectins in livestock. Vet Parasitol 48:109-25. doi:10.1016/0304-4017(93)90149-H CrossRef
  16. Harumal P, Morgan M, Walton SF, Holt DC, Rode J, Arlian LG, Currie BJ, Kemp DJ (2003) Identification of a homologue of a house dust mite allergen in a cDNA library from / Sarcoptes scabiei var. / hominis and evaluation of its vaccine potential in a rabbit/ / S. scabiei var. / canis model. Am J Trop Med Hyg 68: 54-60. http://www.ajtmh.org/content/68/1/54.full.pdf+html
  17. Kemp DJ, Walton SF, Harumal P, Currie BJ (2002) The scourge of scabies. Biologist 49:19-4
  18. Lalli PN, Morgan MS, Arlian LG (2004) Skewed Th1/Th2 immune response to / Sarcoptes scabiei. J Parasitol 90:711-14. doi:10.1645/GE-214R CrossRef
  19. Li BW, Chandrashekar R, Weil GJ (1993) Vaccination with recombinant filarial paramyosin induces partial immunity to / Brugia malayi infection in jirds. J Immunol 150:1881-885
  20. Maroto M, Arredondo JJ, Román MS, Marco R, Cervera M (1995) Analysis of the paramyosin/miniparamyosin gene. Miniparamyosin is an independently transcribed, distinct paramyosin isoform, widely distributed in invertebrates. J Biol Chem 270:4375-382. doi:10.1074/jbc.270.9.4375 CrossRef
  21. Mattsson JG, Ljunggren EL, Bergstr MK (2001) Paramyosin from the parasitic mite / Sarcoptes scabiei: cDNA cloning and heterologous expression. Parasitology 122:555-62. doi:10.1017/S0031182001007648 CrossRef
  22. McCarthy JS, Kemp DJ, Walton SF, Currie BJ (2004) Scabies: more than just an irritation. Postgrad Med J 80:382-87. doi:10.1136/pgmj.2003.014563 CrossRef
  23. Mellanby K (1944) The development of symptoms, parasitic infection and immunity in human scabies. Parasitology 35:197. doi:10.1017/S0031182000021612 CrossRef
  24. Menzano A, Rambozzi L, Rossi L (2007) A severe episode of wildlife-derived scabies in domestic goats in Italy. Small Rumin Res 70:154-58. doi:10.1016/j.smallrumres.2006.02.010 CrossRef
  25. Mounsey KE, Holt DC, McCarthy J, Currie BJ, Walton SF (2008) Scabies: molecular perspectives and therapeutic implications in the face of emerging drug resistance. Future Microbiol 3:57-6. doi:10.2217/17460913.3.1.57 CrossRef
  26. Mounsey KE, McCarthy JS, Walton SF (2013) Scratching the itch: new tools to advance understanding of scabies. Trends Parasitol 29:35-2. doi:10.1016/j.pt.2012.09.006 CrossRef
  27. Nara T, Matsumoto N, Janecharut T, Matsuda H, Yamamoto K, Irimura T, Nakamura KI, Alkawa M, Oswald I, Sher A, Kita K, Kojima S (1994) Demonstration of the target molecule of a protective IgE antibody in secretory glands of / Schistosoma japonicum larvae. Int Immunol 6:963-71. doi:10.1093/intimm/6.7.963 CrossRef
  28. O’Brien D (1999) Treatment of psoroptic mange with reference to epidemiology and history. Vet Parasitol 83:177-85. doi:10.1016/S0304-4017(99)00056-4 CrossRef
  29. Pearce EJ, James SL, Hieny S, Lanar DE, Sher AA (1988) Induction of protective immunity against / Schistosoma mansoni by vaccination with schistosome paramyosin (Sm97), a nonsurface parasite antigen. Proc Natl Acad Sci USA 85: 5678-5682. http://www.pnas.org/content/85/15/5678.full.pdf+html
  30. Pence DB, Ueckermann E (2002) Sarcoptic mange in wildlife. Rev Sci Tech 21:385-98
  31. Purvis RS, Tyring SK (1991) An outbreak of lindane-resistant scabies treated successfully with permethrin 5?% cream. J Am Acad Dermatol 25:1015-016 CrossRef
  32. Roberts LJ, Huffam SE, Walton SF, Currie BJ (2005) Crusted scabies: clinical and immunological findings in seventy-eight patients and a review of the literature. J Infect 50:375-81. doi:10.1016/j.pt.2012.09.006 CrossRef
  33. Rodríguez-Cadenas F, Carbajal-González M, Fregeneda-Grandes J, Aller-Gancedo J, Rodríguez-Cadenas F, Carbajal-González MT, Fregeneda-Grandes JM, Aller-Gancedo JM, Rojo-Vázquez FA (2010) Clinical evaluation and antibody responses in sheep after primary and secondary experimental challenges with the mange mite / Sarcoptes scabiei var. / ovis. Vet Immunol Immunopathol 133:109-16. doi:10.1016/j.vetimm.2009.07.004 CrossRef
  34. Sarasa M, Rambozzi L, Rossi L, Meneguz PG, Serrano E, Granados JE, Gonzalez FJ, Fandos P, Soriguer RC, Gonzalez G, Joachim J, Perez JM (2010) / Sarcoptes scabiei: specific immune response to sarcoptic mange in the Iberian ibex / Capra pyrenaica depends on previous exposure and sex. Exp Parasitol 124:265-71. doi:10.1016/j.exppara.2009.10.008 CrossRef
  35. Solís CF, Ostoa-Saloma P, Lugo-Martinez VH, Johnston SA, Laclette JP (2005) Genetic vaccination against murine cysticercosis by using a plasmid vector carrying / Taenia solium paramyosin. Infect Immunol 73:1895-897 CrossRef
  36. Tang DC, DeVit M, Johnston SA (1992) Genetic immunization is a simple method for eliciting an immune response. Nature 356:152-54. doi:10.1038/356152a0 CrossRef
  37. Tarigan S, Huntley JF (2005) Failure to protect goats following vaccination with soluble proteins of / Sarcoptes scabiei: evidence for a role for IgE antibody in protection. Vet Parasitol 133:101-09. doi:10.1016/j.vetpar.2005.03.044 CrossRef
  38. Vázquez-Talavera J, Solís CF, Terrazas LI, Laclette JP (2001) Characterization and protective potential of the immune response to / Taenia solium paramyosin in a murine model of cysticercosis. Infect Immun 69:5412-416. doi:10.1128/IAI.69.9.5412-5416.2001 CrossRef
  39. Walton SF (2010) The immunology of susceptibility and resistance to scabies. Parasite Immunol 32:532-40. doi:10.1111/j.1365-3024.2010.01218.x
  40. Walton SF, Currie BJ (2007) Problems in diagnosing scabies, a global disease in human and animal populations. Clin Microbiol Rev 20:268-79. doi:10.1128/CMR.00042-06 CrossRef
  41. Walton SF, Myerscough MR, Currie BJ (2000) Studies in vitro on the relative efficacy of current acaricides for / Sarcoptes scabiei var. / hominis. Trans R Soc Trop Med Hyg 94:92-6. doi:10.1016/S0035-9203(00)90454-1 CrossRef
  42. Wang QM, Chen RW, Guo YJ, Sun SH (2000) Study on immune protection elicited by / Cysticerus cellulosae paramyosin (AgB) nucleic acid vaccine. Acad J Sec Mil Med Univ 21:504-07 (in Chinese)
  43. Wang XY, Chen WJ, Lv XL, Tian YL, Men JT, Zhang XF, Lei HL, Zhou CH, Lu FL, Liang C, Hu XC, Xu J, Wu ZD, Yu XB (2012) Identification and characterization of paramyosin from cyst wall of metacercariae implicated protective efficacy against / Clonorchis sinensis Infection. PLoS ONE 7(3):e33703. doi:10.1371/journal.pone.0033703 CrossRef
  44. Yang J, Yang YP, Gu Y, Li Q, Wei JF, Wang SH, Boireau P, Zhu XP (2008) Identification and characterization of a full-length cDNA encoding paramyosin of / Trichinella spiralis. Biochem Biophys Res Commun 365:528-33. doi:10.1016/j.bbrc.2007.11.012 CrossRef
  45. Zalunardo M, Cargill CF, Sandeman RM (2006) Identification of auto-antigens in skin scrapings from scabies-infected pigs. Int J Parasitol 36:1133-141. doi:10.1016/j.ijpara.2006.06.002 CrossRef
  46. Zhao QP, Moon S, Na BK, Kim SH, Cho SH, Lee HW, Kong Y, Sohn WM, Jiang MS, Kim TS (2007) / Paragonimus westermani: biochemical and immunological characterizations of paramyosin. Exp Parasitol 115:9-8. doi:10.1016/j.exppara.2006.05.004 CrossRef
  47. Zhou SH, Liu SX, Song GC, Xu YX, Sun WY (2000) Protective immunity induced by the full-length cDNA encoding paramyosin of Chinese / Schistosoma japonicum. Vaccine 18:3196-204. doi:10.1016/S0264-410X(99)00555-1 CrossRef
  48. Zhu MZ, Xu XM, Liu HW, Liu XJ, Wang S, Dong FT, Yang BL, Song GX (2003) Enhancement of DNA vaccine potency against herpes simplex virus 1 by co-administration of an interleukin-18 expression plasmid as a genetic adjuvant. J Med Microbiol 52:223-28. doi:10.1099/jmm.0.04998-0 CrossRef
  
• 作者单位：Xiaobin Gu (1) (4)
  Yue Xie (1)
  Shuxian Wang (1)
  Xuerong Peng (2)
  Songjia Lai (3)
  Guangyou Yang (1)
  
  1. Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, 625014, China
  4. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China
  2. Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya’an, 625014, China
  3. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, 625014, China
  
• ISSN：1572-9702

文摘

Sarcoptes scabiei is the causal agent of the highly contagious disease sarcoptic mange (scabies) that affects animals and humans worldwide. An increasing number of cases of treatment failure is being reported because of drug resistance. The development of a specific vaccine would be a sustainable option for control of this disease. In this study, we cloned and expressed a S. scabiei gene encoding paramyosin (PAR) and investigated the immune response elicited by DNA encoding PAR in mice. The ability of the DNA vaccine to express antigen in COS-7 cells was confirmed by RT-PCR and IFA. The immune response induced by DNA vaccine was investigated by ELISA, splenocyte proliferation assay, and cytokine production assay. Compared to the pVAX1 control group, the PAR DNA vaccination group showed the higher levels of IgG, IgG1, IgG2a, IgE, IgM, stronger lymphocyte proliferation in mouse spleen, and larger production of IL-2, IL-4, IL-5, and IFN-γ in the supernatant of cultures from splenocytes. These results indicated that the PAR DNA vaccine induced a mixed Th1/Th2 response in mice. In conclusion, our results revealed that the S. scabiei PAR DNA vaccine induced both a humoral and cellular immune response, which would provide basic data for the further study to develop an effective vaccine against sarcoptic mange.